中国药业2025，Vol.34Issue(7)：59-64,6.DOI:10.3969/j.issn.1006-4931.2025.07.012
基于PI3K/Akt信号通路探讨化痰祛瘀方治疗痰瘀互结证冠状动脉粥样硬化性心脏病作用机制
Mechanism of Huatan Quyu Formula in the Treatment of Coronary Heart Disease with Phlegm-Blood Stasis Syndrome Through the PI3K/Akt Signaling Pathway
摘要
Abstract
Objective To investigate the mechanism of Huatan Quyu Formula in the treatment of coronary heart disease(CHD)with phlegm-blood stasis syndrome through the PI3K/Akt pathway.Methods Thirty rats were randomly divided into the blank control(group A),model control(group B),and low-,medium-,high-dose Huatan Quyu Formula groups(groups C1,C2,C3),with six rats in each group.Rats in groups B,C1,C2,and C3 were fed the high-fat diet for 8 weeks,and then they were intraperitoneally injected with 30 U/kg of posterior pituitary hormone to establish CHD models.After 8 weeks of normail diet,rats in group A were intraperitoneally injected with an equal volume of distilled water.The rats in groups C1,C2,and C3 were administered Huatan Quyu Formula at 25,50,and 75 mg/(kg·d)via gavage,respectively,while rats in groups A and B received equal volume of distilled water for 4 weeks.Cardiac function parameters[left ventricular ejection fraction(LVEF),left ventricular end-diastolic diameter(LVEDd),left ventricular end-systolic diameter(LVESd),fractional shortening(FS)]were measured by the echocardiography.Myocardial pathological damage was assessed by the hematoxylin-eosin(HE)staining.The levels of serum lipid indexes[total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C)],and myocardial/endothelial injury markers[endothelin-1(ET-1),creatine kinase-MB(CK-MB),cardiac troponin T(cTnT)]were analyzed by the automatic biochemical analyzer.The levels of inflammatory factors[tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6)]were detected by the enzyme-linked immunosorbent assay(ELISA).The expression levels of B-cell lymphoma 2(Bcl-2),Bcl-2-associated X protein(Bax),cleaved caspase-3(c-caspase-3),phosphatidylinositol-3-kinase(PI3K),phosphorylated PI3K(p-PI3K),serine/threonine-protein kinase(Akt),and phosphorylated Akt(p-Akt)protein were measured by the Western blot.Results Compared with those in group B,the LVEDd and LVESd,the levels of serum TC,TG,LDL-C,CK-MB,cTnT,ET-1,myocardial TNF-α,IL-1β and IL-6,and the expression levels of Bax,c-caspase-3,p-PI3K,p-Akt protein in groups C1,C2 and C3 significantly decreased(P<0.05),while the LVEF and FS,the levels of serum HDL-C,and the expression level of myocardial Bcl-2 significantly increased(P<0.05).The results of HE staining showed that the myocardial cell swelling,myocardial fiber disorder,myocardial cell necrosis,and atrophy in groups C1,C2,and C3 all improved.Conclusion Huatan Quyu Formula can improve cardiac function,lipid profiles and myocardial/endothelial injury,inhibit apoptosis,and alleviate inflammatory reactions in CHD model rats.Its mechanism may be related to the inhibition of PI3K/Akt signaling pathway activation.关键词
化痰祛瘀方/冠状动脉粥样硬化性心脏病/痰瘀互结证/磷脂酰肌醇-3-激酶/丝氨酸/苏氨酸蛋白激酶Key words
Huatan Quyu Formula/coronary heart disease/phlegm-blood stasis syndrome/phosphatidylinositol-3-kinase/serine/threonine-protein kinase分类
医药卫生引用本文复制引用
韩佳,杜鸿瑶,鲁静,潘晔..基于PI3K/Akt信号通路探讨化痰祛瘀方治疗痰瘀互结证冠状动脉粥样硬化性心脏病作用机制[J].中国药业,2025,34(7):59-64,6.基金项目
河北省中医药管理局中医药类科研计划课题[2022548]. （）
