中国药业2025，Vol.34Issue(7)：65-68,4.DOI:10.3969/j.issn.1006-4931.2025.07.013
超高效液相色谱法同时测定伤科跌打片大黄中5种游离蒽醌含量
Simultaneous Determination of Five Free Anthraquinones in Rhei Radix et Rhizoma from Shangke Dieda Tablets by UPLC
摘要
Abstract
Objective To establish an ultra-high performance liquid chromatography(UHPLC)method for the simultaneous determination of aloe-emodin,rhein,emodin,chrysophanol,and physcion in Rhei Radix et Rhizoma from Shangke Dieda Tablets.Methods The chromatographic column was Agilent Poroshell 120 SB-C18 column(100 mm×4.6 mm,2.7 µm),the mobile phase was methanol-0.1%phosphoric acid solution(85∶15,V/V),the flow rate was 1.6 mL/min,the column temperature was 30℃,the detection wavelength was 254 nm,and the injection volume was 2 µL.Results The linear ranges of aloe-emodin,rhein,emodin,chrysophanol,and physcion were 0.377 5-56.628 0 µg/mL,0.468 3-70.244 8 µg/mL,0.438 1-65.721 6 µg/mL,0.431 4-64.709 4 µg/mL,and 0.164 7-24.709 2 µg/mL(r=0.999 9,n=6),respectively.The RSDs of precision,stability,and repeatability tests were all lower than 2.5%(n=6).The average recoveries of aloe-emodin,rhein,emodin,chrysophanol,and physcion were 97.11%,102.73%,105.00%,96.30%,and 105.12%,with RSDs of 1.28%,1.69%,1.87%,1.81%,and 1.16%(n=6),respectively.The average total content of the five components in 74 batches of Shangke Dieda Tablets from 10 manufacturers was 0.59 mg/tablet,exceeding the specified limit of 0.24 mg/tablet.Conclusion The method is simple,rapid,and accurate,which can be used for quality control of Shangke Dieda Tablets.关键词
超高效液相色谱法/伤科跌打片/大黄/芦荟大黄素/大黄酸/大黄素/大黄酚/大黄素甲醚/含量测定Key words
UPLC/Shangke Dieda Tablets/Rhei Radix et Rhizoma/aloe-emodin/rhein/emodin/chrysophanol/physcion/content determination分类
医药卫生引用本文复制引用
唐雪婷,王兆琦,田金苗,孔令锋,周春玲..超高效液相色谱法同时测定伤科跌打片大黄中5种游离蒽醌含量[J].中国药业,2025,34(7):65-68,4.基金项目
2023年国家药品评价性抽验项目[67]. （）
