中国医药科学2025，Vol.15Issue(5)：4-8,5.DOI:10.20116/j.issn2095-0616.2025.05.01
电针通过调节TREM2-DAP12信号通路改善大鼠术后认知功能的机制研究
Research on the mechanism of electroacupuncture improving postoperative cognitive function in rats by regulating TREM2-DAP12 signal pathway
摘要
Abstract
Objective To investigate the mechanism of electroacupuncture regulating the signal pathway of triggering receptor expressed on myeloid cells 2(TREM2)-DNAX activating protein of 12 kDa(DAP12)to improve the postoperative cognitive function of tibial fracture in aged rats.Methods A total of 20 healthy male specific pathogen free(SPF)rats aged 18 months were divided into the electroacupuncture group(n=10)and the control group(n=10)according to the random number table method.The electroacupuncture group was stimulated by Baihui(GV20)and Shenting(GV24)electroacupuncture,while the control group was not stimulated by electroacupuncture.The cognitive function of rats was evaluated by open field test.Hematoxylin-eosin staining was used to observe the morphology of hippocampal cells.Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL)staining was used to observe the apoptosis of hippocampus.The plasma concentrations of amyloid β-protein(Aβ)1-40,interleukin(IL)-1β,IL-6 and tumor necrosis factor(TNF)-α were measured by enzyme-linked immunosorbent assay(ELISA).The expression of neuron-specific enolase(NSE),S-100β protein,TREM2 and DAP12 protein in hippocampus were detected by Western blot.Results Cognitive function:Compared with the control group,the activity time,total distance and stretching time of rats in the electroacupuncture group were all significantly prolonged,and the average speed was significantly increased(P<0.05).Morphology and apoptosis of hippocampal cells:In the control group,the nuclei of neurons in cornu ammonis area 3(CA3)were condensed and the cytoplasm was deeply stained,and microglia phagocytosis neurons could be seen.In the electroacupuncture group,only some cells in CA3 area were deeply stained.Compared with the control group,the number of neuronal apoptosis in the electroacupuncture group decreased remarkably,with statistically significant difference(P<0.05).Plasma concentrations of Aβ1-40,IL-1β,IL-6 and TNF-α:Compared with the control group,the expression levels of Aβ1-40,IL-1β,IL-6 and TNF-α of rats in the electroacupuncture group were remarkably decreased,with statistically significant differences(P<0.05).Expression levels of NSE,S-100β,TREM2 and DAP12 in hippocampus:Compared with the control group,the expression levels of NSE and S-100β protein in the electroacupuncture group decreased remarkably,with statistically significant differences(P<0.05),while the expression levels of TREM2 and DAP12 increased remarkably,with statistically significant differences(P<0.05).Conclusion Electroacupuncture stimulation of Baihui(GV20)and Shenting(GV24)can alleviate the injury and apoptosis of hippocampal neurons after tibial fracture surgery in aged rats,and reduce neurocognitive impairment,which may be related to electroacupuncture activating TREM2-DAP12 pathway,promoting the transformation of microglia from M1 type to M2 type,and alleviating the inflammatory reaction of microglia.关键词
围手术期神经功能紊乱/大鼠/电针/TREM2-DAP12信号通路/小胶质细胞Key words
Perioperative neurocognitive disorder/Rats/Electroacupuncture/TREM2-DAP12 signal pathway/Microglia分类
医药卫生引用本文复制引用
王翔锋,李紫嫣,罗颖,蒋思敏,裴益聪,刘威..电针通过调节TREM2-DAP12信号通路改善大鼠术后认知功能的机制研究[J].中国医药科学,2025,15(5):4-8,5.基金项目
国家自然科学基金项目基础提升计划专项课题(JCZX202306). （JCZX202306）
