Abstract
Objective To establish an inductively coupled plasma-mass spectrometry(ICP-MS)method for simultaneous determination of 19 inorganic elements[potassium(K),calcium(Ca),sodium(Na),magnesium(Mg),aluminum(Al),zinc(Zn),iron(Fe),manganese(Mn),selenium(Se),cadmium(Cd),lead(Pb),copper(Cu),boron(B),Cr(Cr),barium(Ba),mercury(Hg),molybdenum(Mo),cobalt(Co),and arsenic(As)]in Glechomae Herba,and to determine the chemical stoichiometry analysis method for the distribution characteristics of inorganic elements.Methods Microwave digestion was used for sample pre-treatment,the flow rate was set at 17 L/min for the plasma argon and 1.5 L/min for the carrier gas,the sampling depth was 11 mm,the temperature of the atomization chamber 3.0℃,the measurement mode was collision pool kinetic energy discrimination(KED)mode,the collision airflow was 6.5 mL/min,the injection flushing time was 45 s,and the peristaltic pump speed was 60 r/min.With scandium(45Sc),indium(115In),and bismuth(209Bi)as internal standards,the fingerprints of inorganic elements were established,and principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were used to evaluate its characteristic elements.Results The linear relationship between the response values of 19 inorganic elements within their respective mass concentration ranges and the measured elements/internal standard elements was good(r≥0.999 0).The RSDs of precision,repeatability,and stability test results were all lower than 4%(n=6).The recovery rates of inorganic elements were in the range of 80.69%-99.85%,with RSDs of 0.92%-4.03%(n=6).Among the 20 batches of samples,the they mainly contained constant elements Ca,Na,Mg,K(>45 mg/kg),the elements with a high content of trace elements were Fe,Zn,Al,Ba,B,Mo,while the heavy metals and harmful elements Pb,As,Cd,Cu did not exceed the standard,and Co and Hg elements were not detected.The fingerprints of inorganic elements were generally similar,among which Ca,Zn,Fe,Cu,and B were the quality differential components of different batches of Glechomae Herba.Conclusion This method is simple,accurate,and has good reproducibility,which can be used for the determination and quality evaluation of 19 inorganic elements in Glechomae Herba.关键词
连钱草/电感耦合等离子体质谱法/主成分分析/正交偏最小二乘法判别分析/无机元素/特征图谱Key words
Glechomae Herba/ICP-MS/PCA/OPLS-DA/inorganic elements/fingerprints分类
医药卫生
