中国农业科技导报2025，Vol.27Issue(4)：22-35,14.DOI:10.13304/j.nykjdb.2024.0620
小麦花粉孔发育相关TaINP1基因鉴定及表达分析
Identification and Expression Analysis of TaINP1 Gene Related to Pollen Pore Development in Wheat
摘要
Abstract
Pollen pore size regulators play central roles in the formation of plant germination pore size.To study the regulation of germination pore development on the pollen surface of wheat and explore the regulatory function of the TaINP1 gene family homologous to OsINP1 in the formation of germination pore size in wheat,53 members of wheat TaINP1 gene family were identified in the wheat genome using bioinformatics strategies,and their physicochemical properties,promoter cis acting elements and collinearity relationship between genes were systematically analyzed.Tissue expression analysis showed that TaINP2.2 and TaINP2.5 genes were significantly expressed in panicle of wheat,some TaINP1 genes,such as TaINP2.5 and TaINP3.2 etc,were highly induced in pollen after low temperature(10℃)treatment.Combined with pollen germination and nano-magnetic bead conversion technology,the RFP reporter gene was successfully introduced into wheat pollen,and the fluorescence intensity of pollen at different low temperatures was observed by laser confocal microscopy,the results showed that the TaINP1 genes played important roles in regulating pollen pore opening and closing.Above results provided basis for the regulation mechanism of wheat pollen pore,and also provided reference for optimizing the efficient transformation system of wheat pollen nanobeads,and also opened up a new way for genetic improvement of wheat molecular breeding.关键词
小麦/花粉孔发育/INP1基因家族/表达模式/低温Key words
wheat/pollen development/INP1 gene family/expression patterns/conversion solution/low temperature分类
农业科技引用本文复制引用
马蓓,公杰,杜银柯,甘雨薇,程蓉,朱波,易丽霞,马锦绣,高世庆..小麦花粉孔发育相关TaINP1基因鉴定及表达分析[J].中国农业科技导报,2025,27(4):22-35,14.基金项目
北京市科技新星交叉项目(20220484204) （20220484204）
北京市农林科学院创新能力建设专项(KJCX20230203) （KJCX20230203）
农业生物育种国家科技重大专项(2023ZD040230204) （2023ZD040230204）
作物表型组学协同创新中心项目(KJCX20240406). （KJCX20240406）
