植物营养与肥料学报2025，Vol.31Issue(3)：578-587,10.DOI:10.11674/zwyf.2024348
接种兰科菌根真菌对不同硝铵比条件下大花蕙兰生长、养分吸收及氮代谢的影响
Effect of orchid mycorrhizae fungus inoculation on the growth,nutrient absorption,and nitrogen metabolism of Cymbidium hybridum under different nitrate-to-ammonium ratios
摘要
Abstract
[Objectives]This study is to explore the impact of orchid mycorrhizal fungi(OMF)inoculation on the growth and nitrogen metabolism of Cymbidium hybridum under various NO3-/NH4+ratios.[Methods]Cultivar Cymbidium hybridum ʻ580ʼ and OMF C2y1 were used as test materials in a pot experiment.The C2y1 inoculation was carried out by watering into soils during the seedling stage of Cymbidium hybridum,taking water as control group.Under each group,N was supplied in three different NO3-/NH4+ratios of 8∶1(T1),1∶1(T2),and 1∶8(T3).The increased growth and biomass weight of the shoot,and the enhanced length of roots of C.hybridum seedlings were measured,the nutrient contents in both shoot and root,and the activities of nitrate reductase(NR)and glutamine synthetase(GS)in leaves were analyzed.PCR method was used to confirm the infection of C2y1 fungi.[Results]Without inoculation,the significantly higher fresh weight growth and dry matter accumulation of C.hybridum were recorded under T2,while the significantly higher root growth was recorded at T3.The PCR results proved the infection of C2y1 on the root of C.hybridum through the soil watering method,and the infection intensity was enhanced with the increase of ammonia nitrogen supply ratio.Compared to the no inoculation control,C2y1 inoculation significantly enhanced shoot growth and biomass accumulation of C.hybridum under various nitrogen form ratios,with the largest promotion on shoot growth under T3 treatment,and the highest promotion on root was under T1 treatment.C2y1 inoculation significantly enhanced the absorption of N,P,K,Fe,Zn,Cu.Notably,with the highest concentrations of N,P,K,and Zn in shoot under T1 ratio,while their concentration in roots were not affected by C2y1 inoculation.The NR activity in C.hybridum seedlings under C2y1 inoculation was positively correlated with the nitrate nitrogen supply ratios,while GS activity was on the contrary.[Conclusions]C.hybridum prefers nitrate to ammonia,however,ammonia is conducive the infection of C2y1,which help C.hybridum to adapt to the soil environment.Inoculation of C2y1 could stimulate the growth and nutrient absorption,and the promotion effect under high ammonia environment is even more obvious.关键词
大花蕙兰/兰科菌根真菌/硝铵比/矿质营养Key words
Cymbidium hybridum/orchid mycorrhizal fungus/nitrate to ammonium nitrogen ratio/mineral nutrition引用本文复制引用
刘舒,杨菲,廖庆刚,赵小兰..接种兰科菌根真菌对不同硝铵比条件下大花蕙兰生长、养分吸收及氮代谢的影响[J].植物营养与肥料学报,2025,31(3):578-587,10.基金项目
国家自然科学基金项目(31170654) （31170654）
惠州学院教授、博士科研启动项目(2019JB039). （2019JB039）
