中国农业科技导报2025，Vol.27Issue(4)：45-56,12.DOI:10.13304/j.nykjdb.2024.0059
陆地棉GhCOMT28对干旱胁迫的响应
Response of GhCOMT28 to Drought Stress in Gossypium hirsutum
摘要
Abstract
Melatonin,as an indoleamine compound,has a wide range of roles in regulating plant growth and development and resisting adversity stress.COMT(caffeic acid O-methyltransferase)is an O-methyltransferase that plays an important role in the phenylpropane metabolic pathway and is a key enzyme in melatonin synthesis.There are 57 GhCOMTs homologous genes in terrestrial cotton,among which GhCOMT28 and GhCOMT55 have the highest homology with Arabidopsis thaliana AtCOMT.In this study,GhCOMT28 was used as an example to parse its biological function,and GhCOMT28 and GhCOMT55 had the highest expression in the roots and stems of cotton,and GhCOMT28 was localized in the cells in the cytoplasm of cell.GhCOMT28 mRNA was induced by drought stress,and silencing of GhCOMT28(TRV2:GhCOMT28)resulted in reduced melatonin levels in leaves,and the expression of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)and other antioxidant enzymes decreased,free radicals accumulation increased,and plant drought tolerance decreased.Overexpression of GhCOMT28(35S:GhCOMT28-GFP)in Arabidopsis significantly improved plant drought tolerance.This study revealed that GhCOMT28 had an important role in regulating drought tolerance in cotton seedlings and had potential use for drought-tolerant variety improvement.关键词
棉花/褪黑素/GhCOMT28/非生物胁迫/干旱胁迫Key words
cotton/melatonin/GhCOMT28/abiotic stress/drought stress分类
农业科学引用本文复制引用
陈宜新,杨秀波,田士军,王聪,白志英,李存东,张科..陆地棉GhCOMT28对干旱胁迫的响应[J].中国农业科技导报,2025,27(4):45-56,12.基金项目
河北省自然科学基金项目(C2022204036,C2023204302) （C2022204036,C2023204302）
河北省省级科技计划资助项目(23567601H) （23567601H）
河北省高等学校科学技术研究项目(QN202037) （QN202037）
华北作物改良与调控国家重点实验室自主研究课题项目(NCCIR2023ZZ-7) （NCCIR2023ZZ-7）
国家自然科学基金项目(31900623). （31900623）
