中国农业科技导报2025，Vol.27Issue(4)：78-86,9.DOI:10.13304/j.nykjdb.2023.0808
葡萄基因组密码子偏好性分析
Analysis of Codon Preference in Vitis vinifera Genome
摘要
Abstract
Codon preference is present in the genomes of almost all known species.In order to understand the codon use preference of Vitis vinifera genome and improve the efficiency of gene heterologous expression,the characteristics and influencing factors of coding gene codons in Vitis vinifera were studied and analyzed using genome-wide data of Vitis vinifera as the basic material,and the codon preference were compared with other expression systems and plant genomes of Vitis vinifera subfamily.The results showed that,based the chromosome sequence of grape,21 887 coding sequences were screened out for analyzing the codons preference.The GC content of the codon in encoding gene of Vitis vinifera showed GC1(50.19%)>GC3(43.92%)>GC2(40.36%),and the last base of codon was preferred to A/U.The 19 optimal codons were identified,including GCA,GCU,GAU,UUU,GGA,GGU,CAU,AUU,CUU,AAU,CCA,CCU,CAA,UCA,UCU,ACA,ACU,GUU,UAU,among which 6 codons ended with A and 13 codons ended with U.The preference of Vitis vinifera genomic codon was influenced by natural selection and mutation,and the natural selection was the main factor.Above results provided a theoretical basis for the study of grape genomics,and also provided guidance for improving the expression of heterologous proteins in Vitis vinifera and the in vitro expression of Vitis vinifera-related proteins.关键词
葡萄/遗传密码/密码子偏好性/自然选择/突变压力Key words
Vitis vinifera/genetic code/codon preference/natural selection/mutation pressure分类
农业科技引用本文复制引用
答梁材,刘珊珊,刘俊林,张丽娟..葡萄基因组密码子偏好性分析[J].中国农业科技导报,2025,27(4):78-86,9.基金项目
甘肃省科技计划项目(23JRRA719) （23JRRA719）
西北民族大学引进人才项目(xbmuyjrc202222,xbmuyjrc202223). （xbmuyjrc202222,xbmuyjrc202223）
