浙江农业学报2025，Vol.37Issue(2)：288-299,12.DOI:10.3969/j.issn.1004-1524.20240151
基于叶绿素荧光参数的苗用型大白菜耐热性综合评价体系
Comprehensive evaluation system for heat tolerance of seedling-edible Chinese cabbage(Brassica rapa L.ssp.)based on chlorophyll fluorescence parameters
摘要
Abstract
The heat tolerance evaluation equations for Chinese cabbage(Brassica rapa L.ssp.)based on antioxidant enzyme activities and physiological indicators such as contents of soluble sugars,malondialdehyde,proline,etc.are complicated,time-consuming and labor-intensive,thus a simple and rapid comprehensive evaluation method need to be established.In the present study,33 Chinese cabbage germplasm resources were used as materials,treated at 40℃for 20 h,and 11 indexes such as chlorophyll fluorescence parameters[optimal/maximal quantum yield of PSⅡ(Fv/Fm),minimal fluorescence(Fo),maximal fluorescence(Fm),PSII efficiency coefficient(Fq′/Fm′),elec-tron transport rate(ETR),light-adapted maximum fluorescence(Fm′),non-photochemical quenching(NPQ),chlorophyll index(Chlidx),total chlorophyll content(Chl)]as well as fresh weight(FW)and relative electrical conductivity(REC)were measured after the high-temperature treatment.The principal component analysis and the membership function analysis were used to evaluate the heat tolerance of the tested Chinese cabbage species.The re-sults obtained by the two comprehensive evaluation methods were consistent,and were consistent with the results of heat tolerance phenotype of Chinese cabbage under high temperature.Finally,the regression equation D4=0.464VETR+0.226VChlidx+0.262VChl+0.191VNPQ+0.192Fm′+0.127mFW for the comprehensive evaluation of heat tolerance of Chinese cabbage based on the chlorophyll fluorescence parameters was established through the stepwise regression analysis.Through the above research,a comprehensive evaluation system for heat tolerance of Chinese cabbage that is simple,fast and convenient for measurement has been established.The research results provide tech-nical support for the subsequent screening of heat-tolerant germplasm,breeding of heat-tolerant germplasm and explo-ration of heat tolerance mechanisms of Chinese cabbage.关键词
大白菜/高温/叶绿素荧光参数/主成分分析/隶属函数分析/耐热性Key words
Brassica rapa L.ssp./high temperature/principal component analysis/membership function analysis/chlorophyll fluorescence parameter/heat tolerance分类
农业科技引用本文复制引用
谈静如,胡齐赞,岳智臣,陶鹏,雷娟利,李必元,赵彦婷,臧运祥..基于叶绿素荧光参数的苗用型大白菜耐热性综合评价体系[J].浙江农业学报,2025,37(2):288-299,12.基金项目
浙江省"领雁"研发攻关计划项目(2022C02030) （2022C02030）
浙江省农业新品种选育重大科技专项(2021C02065-5-2) （2021C02065-5-2）
