中国农业科技导报2025，Vol.27Issue(4)：157-168,12.DOI:10.13304/j.nykjdb.2023.0881
蓝莓多糖水提物脱蛋白工艺研究
Study on Deproteinization Process of Blueberry Polysaccharides Water Extract
摘要
Abstract
Using frozen blueberry as raw material,the deproteinization process of blueberry polysaccharides obtained by ultrasound assisted hot water extraction was studied.Taking polysaccharide retention rate,protein removal rate and data weighted average method as the evaluation indexes,4 kinds of deproteinization methods including adsorption method,adsorption-salting out method,adsorption-papain-salting out method,adsorption-trichloroacetic acid-salting out method were comparatived the deproteinization effects of crude polysaccharides water extracts from blueberry.At the same time,on the basis of single factor experiment,orthogonal experiment was used to optimize the deproteinization process conditions of crude polysaccharides water extracts from blueberry by adsorption method.The results showed that the deproteinization effect of the adsorption method was the best,the best process conditions for the deproteinization of adsorption method were that the deproteinization temperature was 50℃,the sample pH was 3.0,the deproteinization time was 40 min,activated carbon dosage was 1.5%.At this condition,the polysaccharide retention rate was 70.22%,the deproteinization rate was 87.88%,and the comprehensive score was 94.79 points.The adsorption method selected in this study had practical operability,and could effectively remove the protein in the crude polysaccharide of blueberry.Above results provided data supports for further research on separation and purification,chromatographic analysis,structure identification,structure-activity relationship and biological activity research and development of polysaccharides.关键词
蓝莓/多糖/脱蛋白/吸附法Key words
blueberry/polysaccharides/deproteinization/adsorption method分类
轻工纺织引用本文复制引用
翁霞..蓝莓多糖水提物脱蛋白工艺研究[J].中国农业科技导报,2025,27(4):157-168,12.基金项目
辽宁省教育厅科学研究经费项目(面上项目)(2021LJKZ1164) （面上项目）
辽宁省天然产物活性分子开发及利用重点实验室科研项目(LZ202308). （LZ202308）
