浙江农业学报2025，Vol.37Issue(2)：311-320,10.DOI:10.3969/j.issn.1004-1524.20240346
基于WGCNA分析CPPU和TDZ对天工墨玉葡萄香气影响及关键基因挖掘
Analysis on the effects of CPPU and TDZ on the aroma of Tiangong Moyu grape using WGCNA and the exploration of the key genes
摘要
Abstract
In order to study the effect of treatment agents on the aroma of seedless grapes,the early-maturing seed-less grape variety Tiangong Moyu was used as the experimental material,and the inflorescence of Tiangong Moyu was treated with forchlorfenuron(CPPU)and thidiazuron(TDZ)combined with gibberellin(GA3).The aroma compo-nents in mature grape fruits were determined,and the expression levels of terpenoid metabolism-related genes in fruit development stage were detected.Combined with transcriptome data,the terpenoid components were analyzed by the weighted gene co-expression network analysis(WGCNA),and the genes related to grape aroma formation were screened.The results showed that a total of 35 components were detected in the mature Tiangong Moyu grape fruits,including 13 terpenoids.After CPPU and TDZ treatment,the content of characteristic aroma substances,namely,nerol,geraniol and linalool decreased significantly(P<0.05).The expression of HMGR(HMGR1 and HMGR3),the rate-limiting enzyme gene of terpenoid synthesis mevalonate pathway(MVA),decreased after treatment.The methylerythritol phosphate pathway(MEP)rate-limiting enzyme gene DXR showed a downward-upward trend after CPPU treatment,while it was the opposite in TDZ.WGCNA results showed that linalool,nerol and geraniol were clustered together,which were highly correlated with the turquoise module.The MYB44 gene was significantly down-regulated after treatment,especially after TDZ treatment,which was consistent with the content changes of the three substances.The results provide a theoretical basis for the application of CPPU and TDZ in grape fruit production.关键词
葡萄/香气/氯吡脲(CPPU)/噻苯隆(TDZ)/加权基因共表达网络分析(WGCNA)Key words
grape/aroma/forchlorfenuron(CPPU)/thidiazuron(TDZ)/weighted gene co-expression network a-nalysis(WGCNA)分类
农业科技引用本文复制引用
郑婷,向江,魏灵珠,吴江,程建徽..基于WGCNA分析CPPU和TDZ对天工墨玉葡萄香气影响及关键基因挖掘[J].浙江农业学报,2025,37(2):311-320,10.基金项目
国家自然科学基金(32202414) （32202414）
浙江省农业(果品)新品种选育重大科技专项(2021C02066-6) （果品）
国家现代农业产业技术体系(CARS-29-13) （CARS-29-13）
浙江省"三农九方"农业科技协作计划项目(2024SNJF026,2023SNJF018) （2024SNJF026,2023SNJF018）
