浙江农业学报2025，Vol.37Issue(2)：321-328,8.DOI:10.3969/j.issn.1004-1524.20240504
牡丹PoLPAT2基因的克隆及表达分析
Cloning and expression analysis of peony PoLPAT2 gene
张美莹 1莫倩 2齐秀双 3佟宁宁 4孔凡 4刘政安 4吕长平 5彭丽平6
作者信息
- 1. 湖南农业大学 园艺学院,湖南 长沙 410128||中国科学院 植物研究所 北方资源植物重点实验室,国家植物园,北京 100093
- 2. 湖南农业大学 园艺学院,湖南 长沙 410128||天香源(辽宁)生物科技有限责任公司,辽宁 葫芦岛 125300
- 3. 天香源(辽宁)生物科技有限责任公司,辽宁 葫芦岛 125300
- 4. 中国科学院 植物研究所 北方资源植物重点实验室,国家植物园,北京 100093
- 5. 湖南农业大学 风景园林与艺术设计学院,湖南 长沙 410128||湖南农业大学 湖南省中亚热带优质花木繁育与利用工程技术研究中心,湖南 长沙 410128
- 6. 中国科学院 植物研究所 北方资源植物重点实验室,国家植物园,北京 100093||天香源(辽宁)生物科技有限责任公司,辽宁 葫芦岛 125300
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摘要
Abstract
Lysophosphatidic acid acyltransferase(LPAT)catalyzes the acylation of lysophosphatidic acid(LPA)at the sn-2 position to form phosphatidic acid(PA),which is one of the key rate-limiting enzymes in oil synthesis.Pe-ony(Paeonia suffruticosa)is an emerging woody oil plant.Studying the evolution and expression patterns of the peo-ny LPAT gene family is of great significance for further exploring the mechanisms of oil synthesis in peony seeds.In this study,we cloned the PoLPAT2 gene from peony seeds and analyzed its physicochemical properties,phylogenetic relationships,gene structure,and expression patterns using bioinformatics methods.The results showed that the cod-ing sequence length of PoLPAT2 was 1 194 bp,encoding 397 amino acids,consisting of 20 different amino acids,and located on the plasma membrane.The relative molecular weight of the protein is 44.82 ku,with a molecular for-mula of C2065 H3235 N541 O544 S15,a total of 6 400 atoms,a theoretical isoelectric point of 9.67.It contains 37 phospho-rylation sites and exhibits high aliphatic properties.Classified as a hydrophilic protein,it possesses 3 transmembrane regions,lacks signal peptides,and has an instability index of 43.68,indicating it is an unstable protein.Protein structure prediction indicates that PoLPAT2 protein consists of four secondary structures,namely irregular coil,alpha helix,beta helix,and elongation chain.Phylogenetic analysis show that PoLPAT2 in peony is most closely related to homologs in grapes and walnuts.qRT-PCR results showed that the expression level of this gene was peaked at 105 d post-late seed development,and the overall trend showed a decrease followed by an increase,indicating that PoL-PAT2 may be related to the stage of seed development.This findings provide reference for the functional verification of the PoLPAT2 gene and the improvement of oil content in peony seeds.关键词
牡丹/溶血磷脂酸酰基转移酶(LPAT)/基因克隆/生物信息学分析Key words
peony/lysophosphatidyl acyltransferase(LPAT)/gene cloning/bioinformatics analysis分类
农业科技引用本文复制引用
张美莹,莫倩,齐秀双,佟宁宁,孔凡,刘政安,吕长平,彭丽平..牡丹PoLPAT2基因的克隆及表达分析[J].浙江农业学报,2025,37(2):321-328,8.
