浙江农业学报2025，Vol.37Issue(2)：329-337,9.DOI:10.3969/j.issn.1004-1524.20240096
2个牡丹切花品种核型、花药形态与花粉特性的比较
Comparative analysis of karyotype,anther morphology and pollen characteristics for two va-rieties of tree peony cut flower
摘要
Abstract
In this study,we examined the post-blooming pollen fertility of two cut tree peony varieties,Fengdan and Huangguan,focusing on its relationship among pollen micro-morphology,metabolism,and viability.We employed the chromosome squash technique to observe nuclear karyotypes,micrometer measurements for anther morphology,and scanning electron microscopy for pollen morphology.Additionally,we used liquid culture and spectrophotometry to assess pollen germination rates and related metabolic indicators.Our results indicated that Fengdan was a diploid karyotype and produced approximately 12.62 million rounded pollen grains per flower,characterized by three fissures and a deformity rate of 97.2%.This variety exhibitedover50%germinationat a simulatedtemperature of 25℃.In contrast,Huangguan possessed a triploid karyotype with around 2.88 million abnormally shaped triangular pollen grains per flower,resulting in a zero of germination rate.Relative to Fengdan,Huangguan showed significant reduc-tions in superoxide dismutase activity,ascorbic acid content,and soluble protein levels by 46.7%,39.3%,and 31.8%,respectively.The abnormal pollen morphology and metabolic disorders in Huangguan likely contribute to its germination failure.Overall,Huangguan variety is characterized by low pollen quantity and viability,minimizing the risk of pollen contamination in cut flower arrangements.关键词
牡丹/切花/染色体倍性/核型分析/花粉活力Key words
tree peony/cut flower/chromosome ploidy/karyotyping/pollen vitality分类
农业科技引用本文复制引用
张欢欢,范挺秀,高双成,范丙友,史国安..2个牡丹切花品种核型、花药形态与花粉特性的比较[J].浙江农业学报,2025,37(2):329-337,9.基金项目
国家重点研发计划项目(2018YFD1000400) （2018YFD1000400）
国家自然科学基金(31372098) （31372098）
河南省自然科学基金(162300410075,202000410137) （162300410075,202000410137）
