浙江农业学报2025，Vol.37Issue(2)：338-348,11.DOI:10.3969/j.issn.1004-1524.20240161
叶面喷施褪黑素对甘蓝幼苗干旱胁迫的缓解作用
Mitigative effect of foliar spraying melatonin on drought stress of cabbage seedlings
摘要
Abstract
To investigate the mitigative effect of melatonin(MT)sprayed on leaves and its optimal concentration on drought stress in cabbage(Brassica oleracea L.var.capitata L.)seedlings,this study used the high-altitude cool-weather vegetable variety'Zhonggan 21'as the experimental material.20％polyethylene glycol-6000(PEG)solu-tion was used as the osmotic agent for simulating drought stress.Different concentrations of MT(50,100,150,200 μmol·L-1)were sprayed onto the leaves,and growth and physiological indicators of the cabbage seedlings were measured.The results showed that drought stress significantly inhibited the growth of cabbage seedlings,and spra-ying different concentrations of MT on the leaves could mitigate the growth inhibition caused by drought stress,with the most significant mitigative effect observed at 100 μmol·L-1 MT.Under drought stress,after spraying 100 μmol·L-1 MT,compared to the drought stress control(T0 treatment),the relative chlorophyll content(expressed by SPAD),and contents of chlorophyll a,chlorophyll b,carotenoid,chlorophyll a+b increased by 28.11％,67.04％,80.70％,46.56％,and 70.79％,respectively;photochemical quenching coefficient(qP),maximum photochemical quantum yield(Fv/Fm),actual photosynthetic efficiency[Y(Ⅱ)],electron transport rate(ETR)and maximum fluorescence yield(Fm′)increased by 32.88％,47.29％,53.19％,60.22％and 49.56％,respec-tively;contents of soluble sugars and soluble proteins increased by 147.36％and 150.02％respectively,while non-photochemical quenching coefficient(NPQ),leaf relative electric conductivity and malondialdehyde(MDA)content decreased by 43.65％,45.11％,and 51.09％,respectively;activities of superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)were1.37,1.34,and2.75 times higher than those under drought stress in leaves,and 1.89,1.28,and 2.08 times higher in roots.In conclusion,foliar application of 100 μmol·L-1 MT can signifi-cantly promote the growth of cabbage seedlings and effectively mitigate the damage caused by drought stress to the high-altitude cool-weather vegetable variety'Zhonggan 21'.关键词
甘蓝/外源褪黑素/干旱胁迫/生长/生理/缓解作用Key words
Brassica oleracea L.var.capitata L./exogenous melatonin/drought stress/growth/physiology/miti-gative effect分类
农业科技引用本文复制引用
任元龙,马蓉,王晓卓,张雪艳..叶面喷施褪黑素对甘蓝幼苗干旱胁迫的缓解作用[J].浙江农业学报,2025,37(2):338-348,11.基金项目
国家重点研发计划(2021YFD1600300) （2021YFD1600300）
六盘山区冷凉蔬菜产业关键技术集成研究与应用示范(2021YFD1600302) （2021YFD1600302）
