中兽医医药杂志2025，Vol.44Issue(2)：1-7,7.DOI:10.13823/j.cnki.jtcvm.2025.004
王不留行药用植物麦蓝菜叶片转录组测序分析
Transcriptome sequencing and analysis of medicinal plant Gypsophila vaccaria leaf
摘要
Abstract
To obtain the transcriptomic information characteristics of Semen Vaccariae,this study conducted high-throughput sequencing of Gypsophila vaccaria leaves using the Illumina 6000 platform and assembled all transcripts.The raw data was uploaded to the NCBI database with the accession number of PRJNA1149650.A total of 99 766 unigenes were generated after the removal of redundancy,of which 65 079 were annotated in seven databases.They were 60 784(NR:93.40%),46 761(SwissProt:71.85%),57 269(EggNOG:87.99%),20 073(KEGG:30.84%),57 269(KOG:87.99%),55 514(Pfam:85.30%)and 33 791(GO:51.92%).Transcription factors such as bHLH,bZIP,and MYB might be involved in the biosynthesis of flavonoids secondary metabolites.Additionally,26 243 SSR loci were detected from 99 766 unigenes.These results can provide basic data for the construction of genetic map,SSR marker development,variety breeding,and analysis of secondary metabolic pathways and regulatory mechanisms of flavonoids of G.vaccaria,and also enrich the G.vaccaria gene database.关键词
王不留行/麦蓝菜/转录组/unigene/转录因子/SSR标记Key words
Semen Vaccariae/Gypsophila vaccaria Sm./transcriptome/unigene/transcription factor/SSR marker分类
农业科技引用本文复制引用
张超强,尉明杰,张家银,杨彬,任培荣,景小强,张勇,王恩军..王不留行药用植物麦蓝菜叶片转录组测序分析[J].中兽医医药杂志,2025,44(2):1-7,7.基金项目
甘肃省自然科学基金(22JR11RG225) （22JR11RG225）
河西学院博士科研启动金(KYQD2022013) （KYQD2022013）
甘肃省科技计划项目(23CXNG0006) （23CXNG0006）
张掖市科技计划项目(ZY2022KY01) （ZY2022KY01）
河西学院校长基金创新团队项目(CXTD2022011) （CXTD2022011）
大学生创新创业训练计划项目(202210740073,S202410740028) （202210740073,S202410740028）
