中兽医医药杂志2025，Vol.44Issue(2)：16-21,封2,7.DOI:10.13823/j.cnki.jtcvm.2025.007
药用植物款冬内生真菌的分离、纯化及抗氧化活性研究
Isolation,purification and antioxidant activity of endophytic fungi from medicinal plant Tussilago farfara L.
摘要
Abstract
The purpose of this experiment was to isolate and purify endophytic fungi from the medicinal plant Tussilago farfarfara L.in the Asteraceae family,and study the antioxidant activity of its secondary metabolites.32 strains of Tussilago farfara L.root,stem and leaf endophytic fungi were obtained by tissue separation,plate marking and other separation and purification methods,and the antioxidant activities of the fermentation products of the endophytic fungi of Tussilago farfara L.were preliminarily determined by using DPPH·radical clearance,hydroxyl radical clearance and total reducing force.Among the fermentation products of 32 strains of endophytic fungi from Tussilago farfara L.,DPPH·scavenging activity of the ethyl acetate extract of the fermentation product of strain KD-18 was better,and the DPPH·scavenging rate was 90.86%when the sample concentration was 1 mg/mL.KD-8 fermentation product ethyl acetate extraction site presented a higher·OH removal activity with a scavenging rate of 89.32%at a concentration of 1 mg/mL;total reducibility of the ethyl acetate extracted from the KD-8 fermentation product was strong,and within the concentration range of 0-0.2 mg/mL,the total reducibility was comparable to that of the VC control.DPPH·free radical content in the fermented product of KD-18 ethyl acetate extract exceeded 90%,demonstrating strong antioxidant activity.The endophytic fungi from Tussilago farfara L.and secondary metabolites are abundant,and some of the metabolites present good pharmacological activity,which can provide the basis for the comprehensive development and utilization of the endophytic fungi from Tussilago farfara L..关键词
款冬/内生真菌/分离/纯化/抗氧化活性Key words
Tussilago farfara L./endophytic fungi/separation/purification/antioxidant activity分类
畜牧业引用本文复制引用
杨震发,刘金菊,姬颖,杨宽,张涛锋,杨爱梅..药用植物款冬内生真菌的分离、纯化及抗氧化活性研究[J].中兽医医药杂志,2025,44(2):16-21,封2,7.基金项目
白银市科技计划项目(2024-1-5N) （2024-1-5N）
甘肃省自然科学基金项目(25CXGA061) （25CXGA061）
国家自然科学基金项目(12305384) （12305384）
