中兽医医药杂志2025，Vol.44Issue(2)：22-29,8.DOI:10.13823/j.cnki.jtcvm.2025.008
单因素-正交试验法优化罗汉果藤水提物制备工艺研究
Optimization of preparation process for aqueous extract from Siraitia grosvenorii vine by single factor-orthogonal test
摘要
Abstract
This study aimed to optimize the preparation process for the aqueous extract from the vine of Siraitia grosvenorii.Single-factor experiments were conducted to investigate the effects of extraction times,extraction time,and material-to-liquid ratio on the yield of the aqueous extract and the content of total flavonoids,soluble sugars,total phenols,and total saponins.Based on the results of the single-factor experiments,an orthogonal test was further employed to determine the optimal extraction process,with the extract yield and the content of active components as evaluation indicators.The results indicated that under the conditions of extracting three times,each time for 1.0 hour,with a material-to-liquid ratio of 1∶16(g∶mL),the yield of the aqueous extract from the vine of Siraitia grosvenorii was the highest,reaching 11.92%,and the average contents of total flavonoids,soluble sugars,total phenols,and total saponins were 4.95 mg/g,30.39 mg/g,12.95 mg/g,and 12.55 mg/g,respectively.These findings provide a scientific basis for the comprehensive utilization of Siraitia grosvenorii vine resources and lay the foundation for further pharmacological research and applications.关键词
罗汉果藤/水提物/提取工艺/单因素试验/正交试验Key words
Siraitia grosvenorii vine/aqueous extract/extraction process/single-factor test/orthogonal test分类
畜牧业引用本文复制引用
束道燕,董朕,刘荣荣,曾建国..单因素-正交试验法优化罗汉果藤水提物制备工艺研究[J].中兽医医药杂志,2025,44(2):22-29,8.基金项目
国家重点研发计划项目(2022YFD1801101) （2022YFD1801101）
