中兽医医药杂志2025，Vol.44Issue(2)：54-59,6.DOI:10.13823/j.cnki.jtcvm.2025.012
数字PCR技术在动物疫病检测中的应用
Application of digital PCR technology in animals epidemic disease detection
摘要
Abstract
In recent years,with the advancement of technology,digital PCR(dPCR)is an emerging technology with absolute quantification of target nucleic acids through portioning the reaction mixture into thousands of tiny independent reaction units.dPCR has several advantages like trace DNA detection,high tolerance to inhibitors,high sensitivity and specificity,and good repeatability.Currently,dPCR also present potential applications in the detecting of animal diseases such as detection of early or mixed infections,monitoring of the treatment effects,and absolute quantification of pathogen DNA concentration.Compared with qPCR,the sensitivity and accuracy of dPCR in parasite detection needs to be further improved.Moreover,the high cost also limits the large-scale promotion and application of dPCR technology to a certain extent.However,with the further development of dPCR technology and the reduction of detection costs,dPCR is expected to play a more important role in clinical diagnosis,epidemiological research,and the identification of asymptomatic pathogen detection in animals.关键词
数字PCR技术/动物疫病诊断/定量检测/猪/牛/羊/禽Key words
digital PCR(dPCR)/animal diseases diagnosis/quantitative detection/pig/cattle/sheep/poultry分类
农业科技引用本文复制引用
孟晓琴,赵淑娟,张晓霞..数字PCR技术在动物疫病检测中的应用[J].中兽医医药杂志,2025,44(2):54-59,6.基金项目
兰州市科技计划项目(2023-3-15) （2023-3-15）
海关总署科研项目(2024HK068) （2024HK068）
