中兽医医药杂志2025，Vol.44Issue(2)：69-73,5.DOI:10.13823/j.cnki.jtcvm.2025.009
长沙地区耐药肠球菌在动物性食品生产链的分布特征
Distribution characteristics of antibiotic-resistant Enterococci in the animal-derived food production chain in Changsha
摘要
Abstract
To investigate the antibiotic resistance of Enterococci in the animal-derived food production chain in Changsha,919 samples were collected from 59 sampling sites across farms,slaughterhouses,and markets for Enterococci isolation and mass spectrometry identification.The minimum inhibitory concentrations(MICs)of 10 antimicrobial agents were determined using the broth microdilution method.A total of 276 Enterococci strains were isolated(with the isolation rate of 30.03%).The highest resistance rates were observed for cefoxitin(90.58%)and ceftiofur(85.14%),followed by florfenicol(72.46%),tetracycline(67.39%),gentamicin(67.39%),erythromycin(64.49%),trimethoprim/sulfamethoxazole(48.55%),enrofloxacin(43.84%),linezolid(26.09%),and vancomycin(17.39%).This study analyzed antibiotic resistance and multidrug resistance of Enterococci from farming(including poultry,sheep,pig,and cattle farms),slaughtering,and retail stages.Specifically,the vancomycin resistance rate of ovine-derived Enterococci in the farming stage reached 56.00%,while porcine-derived Enterococci showed 41.94%resistance rate to linezolid.Additionally,Enterococci from slaughtering and retail stages exhibited lower resistance to macrolides,cephalosporins,and amphenicols compared to those from the farming stage.Multidrug-resistant enterococci(MDR)were prevalent across the supply chain,accounting for 88.77%of isolates,with some strains exhibiting resistance to nine antimicrobial classes,representing"super-resistant"Enterococci.These findings indicate that antibiotic-resistant Enterococci have become widespread in pig,poultry,sheep,and cattle farms in Changsha and may contaminate meat products through cross-contamination during slaughtering and retail,posing a potential risk to consumers.These issues require urgent attention from relevant authorities.关键词
生产链/肠球菌/耐药性/养殖/屠宰/销售Key words
production chain/Enterococci/antibiotic resistance/farming/slaughtering/retail分类
农业科技引用本文复制引用
刘建勤,戴伶,王洪亮,张震萍,陈念,曹槊,陈小军..长沙地区耐药肠球菌在动物性食品生产链的分布特征[J].中兽医医药杂志,2025,44(2):69-73,5.基金项目
湖南省教育厅资助科研项目(22B0189) （22B0189）
长沙市动植物疫病预防控制中心资助项目(0123017) （0123017）
