中兽医医药杂志2025，Vol.44Issue(2)：85-92,8.DOI:10.13823/j.cnki.jtcvm.2025.016
参麦咽舒口服液提取工艺优选及含量测定方法的建立
Optimization of extraction process and establishment of content determination method for Shenmai Yanshu oral liquid
摘要
Abstract
The objective was to optimize the extraction process for Shenmai Yanshu oral liquid and establish a high-performance liquid chromatography(HPLC)method for the simultaneous determination of baicalin and arctiin.Box-Behnken response surface methodology was employed to design experiments,with water addition(8-12 times),extraction time(1.5-2.5 h),and extraction frequency(1-3 times)as the factors under investigation.The comprehensive score based on the content of baicalin and arctiin,as well as the yield of extract,was used as the index to optimize the process.HPLC method was established(chromatographic conditions:CAPCELL PAK C18 column,gradient elution with acetonitrile-0.1%phosphoric acid,flow rate of 1.0 mL/min,detection wavelength of 280 nm)for the simultaneous detection of the two components.The results showed that baicalin and arctiin showed good linear relationships in the range of 0.031 5-0.504 0 mg/mL(r=0.999 6)and 0.021 5-0.343 0 mg/mL(r=0.999 9),respectively.The average recovery rates were 100.3%(RSD=1.04%,n=9)for baicalin and 101.3%(RSD=0.73%,n=9)for arctiin.The optimized process was determined to be adding 12 times water,extracting twice,each time for 2.0 h.Validation experiments showed that the comprehensive score(99.32)had a deviation of<2%from the predicted value(98).The established HPLC method is specific and reproducible,which can effectively control the quality of the preparation.The optimized extraction process is stable and controllable,with reasonable process parameters,providing a scientific basis for large-scale production.The research methods can provide reference for the development of similar traditional Chinese medicine compound preparations.关键词
参麦咽舒口服液/提取工艺优选/Box-Behnken响应面法/高效液相色谱法/黄芩苷/牛蒡苷Key words
Shenmai Yanshu oral liquid/optimization of extraction process/Box-Behnken design-response surface method/HPLC/baicalin/arctiin分类
畜牧业引用本文复制引用
孙琳,金文丽,王艳,谢静,王琼,方佳慧,贾君..参麦咽舒口服液提取工艺优选及含量测定方法的建立[J].中兽医医药杂志,2025,44(2):85-92,8.基金项目
嘉峪关市科技计划项目(23-B02) （23-B02）
