植物学报2025，Vol.60Issue(2)：172-185,14.DOI:10.11983/CBB24110
大豆TPS基因家族在盐胁迫下的表达变化及单倍型选择规律分析
Changes in the Expression of the Soybean TPS Gene Family Under Salt Stress and Haplotype Selection Analysis
摘要
Abstract
INTRODUCTION:Trehalose-6-phosphate synthase(TPS)is a key enzyme involved in the synthesis of trehalose and has been reported to participate in regulating photosynthesis,carbohydrate metabolism,growth and development,and stress responses in various species.Currently,reports on TPS genes in soybean are scarce. RATIONALE:TPS is a stable non-reducing disaccharide,whose synthesis,decomposition and regulation not only provide energy for plant,but also play an important role in plant growth and development and stress tolerance.The in-depth study of soybean TPS genes and its relationships with salt stress is of great significance in elucidating the molecular mechanism of soybean salt tolerance and improving soybean yield. RESULTS:This study identified 20 soybean TPS genes and their associated 10 conserved protein motifs in the soybean genome.Molecular analysis of the promoter elements revealed that the TPS gene promoters are rich in stress-responsive elements.After salt stress treatment,the expression of 17 TPS genes changed,with 12 genes up-regulated and 5 genes down-regulated.Haplotype and selection analyses revealed two major allelic variations in TPS8,TPS13,TPS15,TPS17,and TPS18.Notably,variants carrying TPS15H2,TPS 13H2,TPS17H2,and TPS18H2 were significantly enriched in improved cultivars that underwent strong artificial selection. CONCLUSION:This study reveals the molecular characteristics of the soybean TPS gene family,their expression pat-terns under salt stress,and their evolutionary history,providing a theoretical basis and genetic material for further eluci-dating the functions of soybean TPS genes and breeding salt-tolerant soybean varieties.关键词
大豆/海藻糖-6-磷酸合酶/表达分析/耐盐/人工选择Key words
soybean/trehalose-6-phosphate synthase/expression analysis/salt tolerance/artificial selection引用本文复制引用
曹婕,卢秋连,翟健平,刘宝辉,方超,李世晨,苏彤..大豆TPS基因家族在盐胁迫下的表达变化及单倍型选择规律分析[J].植物学报,2025,60(2):172-185,14.基金项目
广东省自然科学基金面上项目(No.2023A1515011668)、黑龙江省自然科学基金(No.LH2021C078)和河北省现代种业科技创新专项(No.22326316D) （No.2023A1515011668）
