植物学报2025，Vol.60Issue(2)：186-203,18.DOI:10.11983/CBB24101
番茄SIWRKY45转录因子在响应低温和干旱胁迫中的功能
Functions of SIWRKY45 in Response to Low-temperature and Drought Stress in Tomato
摘要
Abstract
INTRODUCTION:Tomato(Solanum lycopersicum),a significant warm-season and water-dependent vegetable crop,is extensively cultivated worldwide.Whether grown in open fields or protected environments,tomatoes frequently encounter various environmental stresses,including drought and low temperatures,which significantly impact their yield and quality.Transcription factors play a pivotal role in plant stress responses by modulating the expression of specific target genes,thereby transmitting perceived stress signals downstream.WRKY transcription factors in tomatoes are known to regulate responses to multiple abiotic stresses.However,the specific role of the tomato SIWRKY45 in abiotic stress responses remains unclear. RATIONALE:Studies have demonstrated that WRKY transcription factors play a crucial regulatory role in plant responses to abiotic stress.As an important economic vegetable crop,tomato is susceptible to various environmental stresses during its growth and development.By genetically overexpressing SIWRKY45 in tomato and investigating its function under low-temperature and drought stress conditions,the findings can provide a theoretical foundation for understanding the complex regulatory mechanisms of WRKY transcription factors.Additionally,this research offers valuable candidate genes for breeding stress-resistant tomato varieties. RESULTS:Expression analysis revealed that low-temperature,drought,and abscisic acid(ABA)treatments significantly induced the expression of SIWRKY45.Overexpression of SIWRKY45 enhanced the resistance of tomato plants to drought and low-temperature stresses.Under drought and low-temperature conditions,the photosynthetic indices,antioxidant enzyme activities,and proline(Pro)contents in SIWRKY45 overexpression lines were significantly higher than those in wild-type(WT)plants.Conversely,the accumulation of reactive oxygen species(ROS)and malondialdehyde(MDA)levels in SIWRKY45-OE plants was significantly lower than in WT plants under the same stress conditions.Transcriptome data analysis indicated that SIWRKY45 regulates tomato's response to low-temperature stress primarily by influencing anti-oxidant enzyme activities and stress response pathways.Dual-luciferase assays demonstrated that SIWRKY45 could directly activate the expression of SIPOD1.Furthermore,the interaction between SIWRKY45 and SIWRKY46 was con-firmed through yeast two-hybrid(Y2H)and bimolecular fluorescence complementation(BiFC)assays. CONCLUSION:Our findings demonstrate that SIWRKY45 positively regulates drought resistance and low-temperature tolerance in tomato.Additionally,SIWRKY45 can interact with SIWRKY46 and directly activate the expression of SIPOD1.These results offer valuable insights for further research into the regulatory mechanisms underlying abiotic stress re-sponses and provide potential gene resources for genetic improvement through molecular breeding.关键词
番茄/SIWRKY45/干旱胁迫/低温胁迫/抗氧化酶Key words
tomato/SIWRKY45/drought stress/low-temperature stress/antioxidase引用本文复制引用
樊蓓,任敏,王延峰,党峰峰,陈国梁,程国亭,杨金雨,孙会茹..番茄SIWRKY45转录因子在响应低温和干旱胁迫中的功能[J].植物学报,2025,60(2):186-203,18.基金项目
延安大学博士科研启动项目(No.YDBK2019-42)、陕西省自然科学基础研究计划(No.2022JQ-159)和陕西省大学生创新创业训练计划(No.S202310719073) （No.YDBK2019-42）
