植物学报2025，Vol.60Issue(2)：204-217,14.DOI:10.11983/CBB24093
基于根系表型性状的蚕豆耐盐碱性鉴定与综合评价
Identification and Comprehensive Evaluation of Faba Bean Salt-alkali Tolerance Based on Root Phenotypic Traits
摘要
Abstract
INTRODUCTION:Identification of salt-alkali tolerant germplasm in faba bean lays the foundation for the exploration of salt-alkali tolerant genes and for the selection and breeding of salt-alkali tolerant varieties,which is of great significance for the utilization of saline-alkali land. RATIONALE:Most of the studies on the salt-alkali tolerance of faba beans were focused on the morphological and physiological traits of the aboveground parts,while there are few studies on the phenotypic traits of the roots.The root plays a crucial role in resisting salt-alkali stress.Deep research on the relationship between the phenotypic traits of the root and the salt-alkali tolerance of faba beans will help to comprehensively understand the physiological mechanism of the salt-alkali tolerance. RESULTS:The results showed that:(1)Under salt-alkali stress,the root overlap number was mostly affected,it was followed by the coila number,while the average diameter of root was affected slightly;(2)Between the overlap number and total number of connection points,among the bifurcation number and the overlap number and total number of con-nection points,most of indicators were significant positively correlation(P＜0.01),while there were significant negatively correlation(P＜0.01)among the average diameter of root and total number of root,the number of root tip,total length of root,endpoint number,coila number,linking number,bifurcation number,overlap number and total number of connection points;(3)Total root surface area of root,total projected area of root,total length of root and total volume of root could be used as the key indicators to identify the salt-alkali tolerance of faba bean during the germination period;(4)Two salt-alkali tolerant accessions H0000809 and H0000653,and two salt-alkali sensitive accessions H0001714 and H0002622 were screened out;and(5)The 399 faba bean accessions were divided into 4 groups:group Ⅰ,salt-alkali to-lerance germplasm,accounting for 0.75％;group Ⅱ,moderately salt-alkali tolerant germplasm,accounting for 8％;groupⅢ,weakly saline-tolerant germplasm,accounting for 52.88％;and group Ⅳ,salt-alkali sensitive germplasm,constituting 38.35％. CONCLUSION:Variation and correlation of each index,and the key indicators used to identify salt-alkali tolerance were determined,extreme materials can be selected and used for future study of salt-alkali tolerance mechanisms in faba bean and the excavation of salt-alkali tolerance genes.关键词
蚕豆/萌发期/盐碱胁迫/耐盐碱种质鉴定/综合评价Key words
faba bean(Vicia faba)/germination stage/salt-alkali stress/salt-alkali tolerant germplasm identification/comprehensive evaluation引用本文复制引用
范惠玲,路妍,金文海,王慧,彭小星,武学霞,刘玉皎..基于根系表型性状的蚕豆耐盐碱性鉴定与综合评价[J].植物学报,2025,60(2):204-217,14.基金项目
国家食用豆产业技术体系建设专项(No.CARS-08)、国家自然科学基金(No.42267008)、青海大学两级财政科研实力提升项目-青年创新人才支持计划(No.2025KTSQ12)和青海省重点研发与转化计划(No.2022-NK-109) （No.CARS-08）
