中南林业科技大学学报2025，Vol.45Issue(3)：37-46,10.DOI:10.14067/j.cnki.1673-923x.2025.03.005
桉树叶部病原菌的分子鉴定与叶际拮抗细菌防效评价
Molecular identification of pathogenic bacteria in Eucalyptus leaves and evaluation of the control effect of phyllosphere antagonistic bacteria
摘要
Abstract
[Objective]Eucalyptus is one of the three fast-growing tree species in China,and as one of the four pillars of Guangxi's forestry economy,it has important economic,social and ecological values.With the expansion of planting area,the change of ecological environment and poor management,the occurrence of Eucalyptus leaf diseases is becoming more and more serious,which affects the growth of Eucalyptus and restricts the healthy development of Eucalyptus industry.Eucalyptus grows rapidly,leaf diseases are difficult to detect in the early stage,and after the disaster,the leaves are lost in a large area,the plant growth cycle is shortened,and the growth is slow,causing significant economic losses.In order to identify the diseases pathogenic fungi,enriching the biological control resources of Eucalyptus leaf diseases and formulate a reasonable disease control plan.[Method]Based on the Koch rule to determine the pathogenicity,identify the pathogenic fungi,use morphological characteristics and rDNA-ITS sequence analysis to identify the pathogenic fungi,and use the plate confrontation method to screen the pathogenic antagonistic bacteria from the surface of Eucalyptus leaves,and identify them,and the evaluation of the control efficacy of biocontrol bacteria was also carried out,so as to provide a basis for the biological control of the Eucalyptus leaf diseases.[Result]The results showed that four pathogenic fungi were isolated from the leaves of eucalyptus trees in Da Gui Shan Stateowned forest farm,namely F202310093,F202310094,F202310095 and F202310096,and the leaf disease symptoms caused by them were consistent with the onset symptoms of forest disease.Combined with morphological characteristics and rDNA-ITS sequence analysis,the four fungi were Colletotrichum fructicola,C.cliviicola,C.siemese and Coniella quercicola.The strains B2311001 with obvious antagonism to the above four pathogenic fungi were screened and identified as Bacillus subtillis by plate confrontation method which identified as Bacillus subtilis by morphological and 16s rDNA sequence analysis.The control effect of antagonistic bacteria was better than that of azoxystrobin,and the potted control effect against C.fructicola was 84.01%,followed by C.siemese with 83.67%,and the lowest control effect was Coniella quercicola,with an interforest control effect of 81.33%.C.cliviicola and C.siamense was first reported as a pathogenic fungi of Eucalyptus leaves disease.[Conclusion]In this study,the antagonistic bacteria of eucalyptus leaf pathogens were screened,and the biocontrol resources of Eucalyptus leaf diseases were enriched,and a theoretical basis was provided for the biological control of Eucalyptus leaf diseases.关键词
桉树/病原菌/分离/鉴定/拮抗细菌Key words
Eucalyptus robuasta/pathogens/segregation/identification/antagonistic bacteria分类
农业科技引用本文复制引用
余平福,李星语,王继春,周娟,许诗萍,何苑皞,邓小军,陈晓龙..桉树叶部病原菌的分子鉴定与叶际拮抗细菌防效评价[J].中南林业科技大学学报,2025,45(3):37-46,10.基金项目
广西自筹经费林业科技项目(桂林科研[2022ZC]第17、24号) （桂林科研[2022ZC]第17、24号）
中央财政林草科技推广示范项目([2023]TG10号). （[2023]TG10号）
