中南林业科技大学学报2025，Vol.45Issue(3)：47-58,12.DOI:10.14067/j.cnki.1673-923x.2025.03.006
不同枝龄落叶松枝内生真菌多样性和群落结构分析
Analysis of endophytic fungal diversity and community structure in Larix olgensis branches of different branch ages
摘要
Abstract
[Objective]Shoot blight of larch caused by Neofusicoccum laricinum is a serious threat to larch growth and development,but studies on the community structure and diversity of endophytic fungi in healthy and diseased larch have not been reported.[Method]This study analyzed differences in the structural composition and diversity of endophytic fungal communities in healthy and diseased larch branches of different branch ages(one-year healthy,two-year healthy,three-year healthy,one-year diseased,two-year diseased and three-year diseased)using high-throughput sequencing technology.[Result]Information on larch endophytic fungi was categorized to 5 phylums,24 orders,83 orders,184 families,and 331 genera.The abundance of Bradymyces,Lophiotrema,Arthrocatena and Devriesia increased with branching age;the abundance of Perusta,Endosporium and Didymella decreased with branching age.The dominant phyla of endophytic fungi in healthy and diseased larch branches were Ascomycota and Basidiomycota.After disease,at the phyla level,the abundance of Dothideomycetes,Pucciniomycetes,Orbiliomycetes and Lecanoromycetes decreased significantly and the abundance of Sordariomycetes,Tremellomycetes and Agaricostilbomycetes increased significantly;at the genera level,the abundance of Capnobotryella and Perusta decreased significantly and the abundance of Neofusicoccum and Chionosphaera increased significantly.The alterations in fungal abundance were accompanied by a considerable increase in the abundance of plant pathogen and fungal parasite-undefined saprotroph fungi,as well as a significant decrease in the abundance of plant pathogen-undefined saprotroph fungi.The key population of the healthy larch fungal network as Lophiotrema,Devriesia and Arthrocatena,and key populations of the diseased larch fungal network are Vishniacozyma,Papiliotrema,Chionosphaera,Camptophora,Neofusicoccum and Endosporium were identified through network analysis.In diseased branches,Neofusicoccum laricinum was concentrated in one-year diseased branches.[Conclusion]Neofusicoccum laricinum changed the community structure of endophytic fungi in plants.There are significant differences in the diversity of endophytic fungal community structure between healthy and diseased groups.This study offers a theoretical framework for the management of shoot blight of larch.关键词
落叶松枯梢病/落叶松/高通量测序/内生真菌/群落多样性Key words
shoot blight of larch/Larix olgensis/high-throughput sequencing/endophytic fungi/community diversity分类
农业科技引用本文复制引用
钱莹莹,邓勋,姜奕冰,张瑶,梁军,宋小双..不同枝龄落叶松枝内生真菌多样性和群落结构分析[J].中南林业科技大学学报,2025,45(3):47-58,12.基金项目
国家重点研发计划项目(2021YFD1400300) （2021YFD1400300）
黑龙江省自然科学基金项目(LH2023C105) （LH2023C105）
黑龙江省省属科研院所科研业务费项目(LKSB2024-9,CZKYF2022-1-B031). （LKSB2024-9,CZKYF2022-1-B031）
