磁共振成像2025，Vol.16Issue(3)：18-23,6.DOI:10.12015/issn.1674-8034.2025.03.003
基于影像转录组学颈痛慢性化的中枢跨尺度机制研究
Investigation of central cross-scale mechanisms in the chronification of neck pain via imaging transcriptomics
摘要
Abstract
Objective:To explore the central pathological mechanisms underlying the transition from acute to chronic neck pain using imaging transcriptomics.Materials and Methods:From March 2023 to May 2024,86 patients with acute neck pain and 89 patients with chronic neck pain were recruited from Shanxi University of Chinese Medicine and Shanxi Provincial Hospital of Acupuncture and Moxibustion.Using a 3.0 T MR scanner to collect resting-state functional magnetic resonance imaging(rs-fMRI)data based on blood oxygen level-dependent signals from the subjects,with the hypothalamus as the seed region for whole-brain functional connectivity(FC)analysis.Imaging transcriptomics analysis was performed using the Allen Human Brain Atlas(AHBA)transcriptomic dataset,and partial least squares(PLS)regression was employed to investigate regional changes in brain functional activity and gene expression between chronic and acute pain patients.Gene enrichment analysis was conducted using Metascape to reveal the central cross-scale mechanisms during the chronicification of neck pain.Results:Compared to acute neck pain,patients with chronic neck pain exhibited increased FC values in the left hypothalamus and right orbital superior frontal gyrus,and decreased FC values in the left hypothalamus and right middle frontal gyrus(Voxel-level P<0.01,Cluster-level P<0.05).Additionally,this study found that the PLS1 model explained 43.43%of the variance,and the PLS1weighted gene expression profile was positively correlated with the case-control t-map space(Pearson's r=0.491,P<0.05).Enrichment analysis revealed that PLS1+genes were closely associated with cellular components such as"glutamatergic synapse"and biological processes like"synaptic signaling,"while PLS1-genes were closely related to cellular components like"intermediate filament cytoskeleton,"molecular functions such as"DNA binding transcription activator activity,"and biological processes including"regulation of growth hormone secretion".Conclusions:A cross-scale analysis based on imaging transcriptomics has uncovered comprehensive changes in brain functional activity,gene expression,and cell composition during the chronicification of neck pain.This indicates that the chronicification of neck pain is a multi-level and multi-scale interactive process involving molecular levels,cellular structures,and brain network functions.关键词
颈痛/影像转录组/功能连接/疼痛慢性化/磁共振成像/跨尺度机制Key words
neck pain/imaging transcriptomics/functional connectivity/chronic pain development/magnetic resonance imaging/cross-scale mechanisms分类
医药卫生引用本文复制引用
高珍,崔梦洁,王海军,冀来喜..基于影像转录组学颈痛慢性化的中枢跨尺度机制研究[J].磁共振成像,2025,16(3):18-23,6.基金项目
Shanxi Basic Research Program(No.202203021221195) （No.202203021221195）
Shanxi University of Chinese Medicine Scientific Research Fund for the Doctoral Scholar(No.2023BK17) （No.2023BK17）
Outstanding Doctoral Graduate Returning to Work in Shanxi Incentive Fund for Scientific Research Startup(No.2023BKS12). 山西省基础研究计划项目(编号:202203021221195) （No.2023BKS12）
山西中医药大学博士科研启动基金项目(编号:2023BK17) （编号:2023BK17）
优秀博士毕业生来晋工作奖励经费科研启动基金项目(编号:2023BKS12) （编号:2023BKS12）
