草业科学2025，Vol.42Issue(1)：90-100,11.DOI:10.11829/j.issn.1001-0629.2023-0527
萎缩芽孢杆菌ZY1825对5种牧草病害的拮抗效果及优化发酵条件
Antagonistic effect of Bacillus atrophaeus ZY1825 on five forage diseases and optimization of fermentation conditions
摘要
Abstract
Bacillus atrophaeus ZY1825 is a bacterial strain isolated from desert sandy soil with biocontrol potential.In this paper,through the plate confrontation method,the antagonistic effect of common vetch anthracnose,ryegrass leaf spot,alfalfa spring black stem,barley fusarium root rot,and alfalfa root rot was determined.The physiological and biochemical characteristics were determined,and the fermentation conditions were optimized.The inhibition rates of the bacteria against the five pathogens were 49.29％,47.21％,50.78％,44.28％,and 45.99％,respectively.The strain is Gram-positive bacteria.The optimal fermentation medium was potato 200 g,sucrose 15 g,yeast extract 12 g,magnesium sulfate 5 g,and distilled water 1000 mL.The number of viable bacteria reached the maximum of 9.28 × 109 cfu·mL-1 at 32 ℃ and 210 r·min-1 liquid culture for 24 h,which was 5.91 times higher than that before optimization,and the inhibition rate of Fusarium oxysporum was increased by 33.45％.This study can provide technical parameters and a theoretical basis for the further use of Bacillus atrophaeus ZY1825 to prepare biocontrol agents to control forage diseases.关键词
萎缩芽孢杆菌/生物防治/牧草病害/防治效果/生理生化特性/发酵条件Key words
Bacillus atrophaeus/biological control/grass diseases/control effect/physiological and biochemical characteristics/fermentation conditions引用本文复制引用
梁玉婷,石海春,李惠霞,高有军,张海英,刘永刚..萎缩芽孢杆菌ZY1825对5种牧草病害的拮抗效果及优化发酵条件[J].草业科学,2025,42(1):90-100,11.基金项目
2023甘肃省重点人才项目 （）
甘肃省农业科学院科研条件建设及成果转化项目(2021GAAS21) （2021GAAS21）
甘肃省中小企业创新基金项目(23CXGH0007) （23CXGH0007）
