化工进展2025，Vol.44Issue(3)：1758-1767,10.DOI:10.16085/j.issn.1000-6613.2024-0349
介孔金属有机骨架固定化漆酶及其活性艳蓝KN-R降解性能
Laccase immobilized on mesoporous metal-organic framework and its performance of reactive brilliant blue KN-R degradation
摘要
Abstract
In order to overcome the unstable and unrecyclable problem of free laccase,mesoporous metal-organic framework PCN-333(Al)with high specific surface area and pore size similar to laccase was selected as the carrier of immobilized laccase,and PCN-333(Al)immobilized laccase[Lac/PCN-333(Al)]was prepared through physical adsorption for reactive brilliant blue KN-R(RBBR)degradation.The results showed that Lac/PCN-333(Al)had extremely high laccase loading(688.9mg/g),and its activity recovery was 70.9%.The pores of PCN-333(Al)provided good protection for laccase,and the pH stability,thermal stability and storage stability of Lac/PCN-333(Al)were improved compared with free laccase.Besides,the Michaelis Menten constant(Km)value of Lac/PCN-333(Al)was 97.6μmol/L,which was much lower than that of free laccase,indicating the affinity of laccase for the substrate became higher after being immobilized.Finally,the degradation efficiency of RBBR by Lac/PCN-333(Al)reached 89.0%within 200min when 0.2mL of ABTS(0.2mmol/L)was added,the degradation rate was 4.5 times higher than that of free laccase,and the degradation efficiency maintained 78.5%after repeated use for 5 times,indicating it had good reusability.This study provided a reference for the development of new immobilized laccase and its application in the treatment of dye wastewater.关键词
金属有机骨架/固定化/酶/生物催化/废水Key words
metal-organic framework/immobilization/enzyme/biocatalysis/waste water分类
资源环境引用本文复制引用
张新宇,陶梦滢,于小婷,赵钟兴,赵祯霞..介孔金属有机骨架固定化漆酶及其活性艳蓝KN-R降解性能[J].化工进展,2025,44(3):1758-1767,10.基金项目
国家自然科学基金(22178073,U23A20120) （22178073,U23A20120）
广西自然科学基金(2020GXNSFGA297001). （2020GXNSFGA297001）
