海南医科大学学报2025，Vol.31Issue(7)：490-496,7.DOI:10.13210/j.cnki.jhmu.20241016.002
槲皮素通过SLC7A11/GPX4/ACSL4通路调控铁死亡改善卵巢早衰的机制研究
Study on the mechanism of quercetin regulating iron death through SLC7A11/GPX4/ACSL4 pathway to improve premature ovarian failure
摘要
Abstract
Objective:To evaluate the protective effect of quercetin on premature ovarian failure(POF)and the regulatory mechanism of iron death based on POF mouse model.Methods:During July and August,2024,a total of 30 five-week-old female SPF C57BL/6 mice were randomly assigned to the normal,model,and quercetin group.Besides the normal group,the POF model was established by intraperitoneal injection of cyclophosphamide in the other two groups.Quercetin group was given querce-tin gavage for 21 d,and the other two groups were given the same dose of normal saline.After the intervention,mouse serum was taken for AMH,FSH,E2,T-SOD,and CAT determination,and ovarian tissue was taken for H&E staining,immunohistochem-ical analysis and qRT-PCR detection.Results:Compared to the normal group,the atretic follicles in the model group were signifi-cantly increased,AMH and E2 levels were significantly decreased(P<0.01),FSH levels were significantly increased(P<0.01),T-SOD activity and CAT activity were decreased significantly(P<0.01),SLC7A11 and GPX4 expressions were signifi-cantly decreased(P<0.05),and the expression of ACSL4 was significantly increased(P<0.01).Compared to the model group,atretic follicles were reduced in the quercetin group,mature follicles were observed in ovarian pathological tissue,AMH and E2 levels were significantly increased(P<0.01),and FSH level decreased significantly(P<0.01).Compared to the model group,T-SOD activity and CAT activity in quercetin group were significantly increased(P<0.05);SLC7A11 expression was in-creased,GPX4 expression was increased,and ACSL4 expression was decreased(P<0.05).Conclusion:Quercetin can inhibit iron death by activating SLC7A11/GPX4/ACSL4 pathway,thereby improving ovarian function and sex hormone level in POF mice.关键词
卵巢早衰/小鼠/槲皮素/铁死亡Key words
Premature ovarian failure/Mice/Quercetin/Iron death分类
中医学引用本文复制引用
麦哲芬,张伊,刘杨萍,韩霞..槲皮素通过SLC7A11/GPX4/ACSL4通路调控铁死亡改善卵巢早衰的机制研究[J].海南医科大学学报,2025,31(7):490-496,7.基金项目
This study was supported by National Natural Science Foundation of China(82205159),Guangdong Administration of Traditional Chinese Medicine(20212203),Natural Science Foundation of Shenzhen(JCYJ20230807120506014,JCYJ20210319124337003),and Shenzhen Futian District Health Public Scientific Research Project(FTWS2021008) 国家自然科学青年基金资助项目(82205159) （82205159）
广东省中医药局面上项目(20212203) （20212203）
深圳市自然科学基金面上项目(JCYJ20230807120506014、JCYJ20210319124337003) （JCYJ20230807120506014、JCYJ20210319124337003）
深圳市福田区卫生公益科研项目(FTWS2021008) （FTWS2021008）
