海南医科大学学报2025，Vol.31Issue(7)：497-505,512,10.DOI:10.13210/j.cnki.jhmu.20240904.001
黄精维护线粒体结构功能促进衰老大鼠骨髓内皮祖细胞功能的研究
Promoting effect of Rhizoma Polygonati on the functional activities of endothelial progenitor cells by restoration of mitochondrial function and maintenance of mitochondrial structure
摘要
Abstract
Objective:To dynamically observe the effect of Rhizoma Polygonati(RP)on the senescence degree,the function-al activities,the mitochondrial function and structure of endothelial progenitor cells(EPCs)derived from bone marrow in the natu-ral aging process of rats.The intervention effect of RP was also observed.Methods:A total of 96 rats(18 months)were randomly divided into four groups:an old group,a RP-low-dose group(1 g/kg),a RP-mid-dose group(2 g/kg),and a RP-high-dose group(4 g/kg),another 24 rats(2 months)were used as the young group.All rats were administered by gavage once a day for 12 weeks.At the 4th,8th,12th week,8 rats in each group were anesthetized and euthanized,then EPCs were isolated,cultured and indentified.EPCs senescence was assessed by beta-galactosidase,cellular proliferation,migration,tubule formation were evaluat-ed by CCK-8,transwell chamber,and in vivo angiogenesis kit,respectively.The level of reactive oxygen species(ROS)was as-sessed by flow cytometry,the mitochondrial membrane potential(MMP)was measured by JC-1,and the ATP content was evalu-ated by chemiluminescence;the structure of mitochondria was observed by transmission electron microscopy.Results:In the old group,EPCs senescence increases with increasing week age,accompanied by a significant decrease in proliferation,migration and tubule formation.The level of ROS increased but the ATP content and MMP both decreased(P<0.05).The mitochondria was solidified and there were more autophagosomes.RP treatment could reduce the positive rate of cell senescence,promote the func-tional activities of EPCs,decrease the ROS level,and increase the ATP content and MMP(P<0.05).The mitochondria struc-ture became regular,and a few autophagosomes were observed.Conclusion:RP could promote the functional activities of EPCs in the natural ageing process of rats by maintaining the integrity of mitochondrial structure and restoration of mitochondrial function.关键词
黄精/内皮祖细胞/衰老/细胞功能/线粒体Key words
Rhizoma Polygonati/Endothelial progenitor cells/Senescence/Cellular functions/Mitochondria分类
中医学引用本文复制引用
秦臻,叶利兵,冯静月,石永芳,许键炜..黄精维护线粒体结构功能促进衰老大鼠骨髓内皮祖细胞功能的研究[J].海南医科大学学报,2025,31(7):497-505,512,10.基金项目
This study was supported by the National Natural Science Foundation of China(81860872,82360964)and Research Project on TCM and Ethnic Medicine Science and Technology of Guizhou Provincial Administration of TCM(QZYY-2024-012) 国家自然科学基金资助项目(81860872,82360964),贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2024-012) （81860872,82360964）
