海南医科大学学报2025，Vol.31Issue(7)：506-512,7.DOI:10.13210/j.cnki.jhmu.20240926.001
淫羊藿苷通过调控PI3K/AKT/mTOR通路影响自然衰老小鼠Treg细胞的表达
Icariin affects the expression of Treg cells in naturally aging mice by regulating the PI3K/AKT/mTOR pathway
摘要
Abstract
Objective:To observe the effect of Icariin in delaying skin aging in naturally senescent mice and to preliminarily ex-plore its mechanism of action.Methods:Fifteen-month-old SPF-grade male C57BL/6J mice were randomly divided into the senes-cence group,Icariin low-dose group(5 mg/kg),and Icariin high-dose group(20 mg/kg),with 10 mice in each group.Another 10 one-month-old male C57BL/6J mice were taken as the young control group.After 3 months of feeding,all mice were executed.H&E staining was used to observe the thickness of mouse skin tissues;Masson staining was used to observe the changes of colla-gen fibers;immunohistochemistry was used to detect the expression of Ki67 and p16 in skin tissues;flow cytometry was used to detect the percentage of CD4+FoxP3+Treg in spleen and lymph nodes,as well as immunohistochemistry was used to detect the expression of Foxp3 in skin tissues;and protein Western blot was used to detect tissue protein expression levels of PI3K,AKT,m-TOR,and P70S6K.Results:Compared to the youth group,the thickness of the skin in the aging group of mice was thinner(P<0.01);the content of collagen fibers was reduced(P<0.01);the expression of epidermal Ki67 was reduced(P<0.01);the ex-pression of p16 in skin tissues was increased(P<0.01);the percentage of CD4+Foxp3+Treg in the tissues of spleen and lymph nodes was increased(P<0.01);the expression of Foxp3 in skin tissue was increased(P<0.01).There was no significant change in protein expression of PI3K,AKT and mTOR in skin tissue,and increased protein expression of p-PI3K,p-AKT,p-mTOR,and P70S6K(P<0.01).Compared to the aging group,the skin of mice in the epimedium glycoside administration group was thickened(P<0.01);collagen fiber content was increased(P<0.01);epidermal Ki67 expression was increased(P<0.01);the expression of p16 in skin tissues was decreased(P<0.01);and the percentage of CD4+Foxp3+Treg in the splenic and lymph node tissues was decreased(P<0.05,P<0.01);and Foxp3 expression in skin tissue was reduced(P<0.01).There was no significant change in protein expression of PI3K,AKT,and mTOR in skin tissues,and the protein expression of p-PI3K,p-AKT,p-mTOR,and P70S6K was reduced(P<0.05,P<0.01).Conclusion:Icariin regulates Foxp3 expression through PI3K/AKT/m-TOR signaling pathway to delay skin aging in naturally aging mice.关键词
自然衰老/淫羊藿苷/调节性T细胞/PI3K/AKT/mTORKey words
Natural aging/Icariin/Treg/PI3K/AKT/mTOR分类
中医学引用本文复制引用
邓锋,侯利,王怡,罗亚男,曾巧煌,温涛,刘翠华..淫羊藿苷通过调控PI3K/AKT/mTOR通路影响自然衰老小鼠Treg细胞的表达[J].海南医科大学学报,2025,31(7):506-512,7.基金项目
This study was supported by the Young Scientists Fund of the National Natural Science Foundation of China(82104442,82371597)and Scientific Research Project of Education Department of Hubei Province(Q20221213) 国家自然科学基金青年项目(82104442、82371597) （82104442,82371597）
湖北省教育厅科学研究计划项目(Q20221213) （Q20221213）
