海南医科大学学报2025，Vol.31Issue(7)：522-530,9.DOI:10.13210/j.cnki.jhmu.20250311.001
miR-193b-5p靶向CD44v6调控宫颈癌细胞侵袭、迁移以及上皮-间质转化的机制研究
Study on the mechanism of miR-193b-5p targeting CD44v6 to regulate the invasion,migration and epithelial-mesenchymal transition of cervical cancer cells
摘要
Abstract
Objective:To explore the regulatory effect of microRNA-193b-5p(miR-193b-5p)on the biological behavior of hu-man cervical cancer SiHa cells and its molecular mechanism.Methods:Cervical cancer tissues from 38 patients with cervical can-cer and normal cervical tissues from 38 patients with hysterectomy during the same period were collected,RT-qPCR was used to detect the expression levels of miR-193b-5p in the two tissues.SiHa cells were divided into the control group,the mimics NC group,the miR-193b-5p mimics group,the inhibitors NC group and the miR-193b-5p inhibitors group,after corresponding trans-fection,the expression levels of miR-193b-5p in the cells of each group were detected by RT-qPCR,the proliferation activity,mi-gration and invasion of SiHa cells in each group were detected by CCK-8 method,cell scratch assay and Transwell chamber meth-od,respectively,cell immunofluorescence staining and Western Blot was used to detect the expression of E-cadherin,N-cadherin,Vimentin and other epithelial-mesenchymal transition(EMT)-related proteins in SiHa cells of each group,bioinfor-matics combined with dual luciferase reporter gene experiments predicted and verified the targeted regulatory relationship between miR-193b-5p and leukocyte differentiation antigen 44 splicing variant v6(CD44v6).Results:The relative expression of miR-193b-5p in cervical cancer tissues was significantly lower than that in normal cervical tissues(P<0.05).Compared with the control group and the mimics NC group,the relative expression of miR-193b-5p in SiHa cells in the miR-193b-5p mimics group was significantly upregulated(P<0.05),the cell proliferation activity and cell scratch healing rate were significantly reduced(P<0.05),the number of cell invasion was significantly reduced(P<0.05),the fluorescence staining intensity and relative protein ex-pression of E-cadherin were significantly increased(P<0.05),and the fluorescence staining intensity and relative protein expres-sion of N-cadherin and Vimentin were significantly decreased(P<0.05);compared with the control group and the inhibitors NC group,the relative expression of miR-193b-5p in SiHa cells in the miR-193b-5p inhibitors group was significantly downregulated(P<0.05),the cell proliferation activity and cell scratch healing rate were significantly increased(P<0.05),the number of cell in-vasion was significantly increased(P<0.05),the fluorescence staining intensity and relative protein expression of E-cadherin were significantly reduced(P<0.05),and the fluorescence staining intensity and relative protein expression of N-cadherin and Vimentin were significantly increased(P<0.05).There is a binding site between miR-193b-5p and the 3′-untranslated region(3′-UTR)of CD44v6,and compared with the mimics NC group,transfection of miR-193b-5p mimics into SiHa cells significantly inhibited the relative luciferase activity of CD44v6 wild type(WT)(P<0.05).Conclusion:miR-193b-5p expression is reduced in human cervi-cal cancer tumor tissues.Increasing its expression can inhibit cell proliferation activity,migration,invasion,EMT and other malig-nant behaviors,its molecular mechanism may be related to the targeted inhibition of CD44v6 expression.关键词
宫颈癌/miR-193b-5p/CD44v6/侵袭/迁移/上皮-间质转化Key words
Cervical cancer/miRNA-193b-5p/CD44v6/Invasion/Migration/Epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
热孜宛古丽·约麦尔,买买提·司马义,张晓玲,叶勒丹·马汉,周玲..miR-193b-5p靶向CD44v6调控宫颈癌细胞侵袭、迁移以及上皮-间质转化的机制研究[J].海南医科大学学报,2025,31(7):522-530,9.基金项目
This study was supported by Natural Science Foundation of Xinjiang Uygur Autonomous Region(2022D01C104) 新疆维吾尔自治区自然科学基金(2022D01C104) （2022D01C104）
