核农学报2025，Vol.39Issue(5)：886-896,11.DOI:10.11869/j.issn.1000-8551.2025.05.0886
基于ISSR和ITS分子标记的海南砂仁种质资源遗传多样性评价
Genetic Diversity Analysis of Amomum longiligulare T.L.Wu Germplasm Based on ISSR and ITS Molecular Markers
摘要
Abstract
In order to protect and manage the resources of A.longiligulare,the genetic diversity and differentiation of 118 germplasm samples from 12 geographic populations of A.longiligulare were analyzed with techniques such as Inter-Simple Sequence Repeat(ISSR)and Internal Transcribed Spacer(ITS)using software such as NTSYS,POPGENE,BioEdit,and Clustalx.The results showed that 25 highly polymorphic ISSR primers were screened,resulting in the amplification of 132 loci,of which 93 were polymorphic,with a polymorphism rate of 70.45％.The average values of observed number of alleles(Na),effective number of alleles(Ne),Nei's genetic diversity(H),Shannon diversityinformation index(I)and gene flow(Nm)were 1.164,1.103,0.059,0.087 and 0.271,respectively.Based on ITS sequence alignment information for A.longiligulare,11 haplotypes were detected,but their distribution was uneven across different populations,with only one haplotype detected in several populations.These findings indicate a limited genetic exchange between different geographic populations of A.longiligulare and significant genetic differentiation.The genetic variation among populations is the source of overall genetic diversity,while as the genetic diversity within populations tends to be low.The lack of gene flow between populations has led to inbreeding depression within A.longiligulare populations.This study provides a scientific basis for the conservation,utilization,and sustainable development of A.longiligulare germplasm resources.关键词
海南砂仁/遗传多样性/遗传分化/ISSR/ITS/单倍型Key words
Amomum longiligulare T.L.Wu/genetic diversity/genetic differentiation/ISSR/ITS/haplotype引用本文复制引用
罗凡,瞿云苹,陈振夏,杨涛,范宇光,潘坤..基于ISSR和ITS分子标记的海南砂仁种质资源遗传多样性评价[J].核农学报,2025,39(5):886-896,11.基金项目
海南省自然科学基金(821RC576),海南医学院大学生创新创业训练计划项目(S202211810001),2019校级引进人才经费(XRC190003) （821RC576）
