核农学报2025，Vol.39Issue(5)：897-906,10.DOI:10.11869/j.issn.1000-8551.2025.05.0897
三七叶片人参皂苷成分对灰葡萄孢菌侵染的响应及转录组分析
Response of Ginsenosides in Panax notoginseng Leaves to Botrytis cinerea Infection and Transcriptome Analysis
摘要
Abstract
To explore the molecular mechanisms underlying gray mold disease resistance in Panax notoginseng leaves,ultra performance liquid chromatography was utilized to monitor the dynamic changes in the content of ginsenosides Rd,Rb1,Rc and Rb3 in Panax notoginseng leaves inoculated with Botrytis cinerea.Moreover,transcriptome sequencing technology was further used to analyze the differentially expressed genes.The results showed that the contents of the four ginsenosides in Panax notoginseng leaves increased by 39.02％to 86.56％after 12 hours of inoculation compared to the control.However,their contents decreased after 24 hours of infection,with a reduction rate ranging from 0.31％to 62.51％.Transcriptome sequencing further indicated that compared to the non-inoculated control,the differentially expressed genes in inoculated leaves at both 12 and 24 hours were significantly enriched in the triterpenoid biosynthesis pathway.Specifically,the expressions of phosphomevalonate kinase(PMVK)and hydroxymethylbutene-4-phosphate synthase(HDS),which were involved in the biosynthesis pathway of ginsenosides in Panax notoginseng leaves,were upregulated at 12 hours post-inoculation with Botrytis cinerea,leading to an increase in ginsenoside content.However,the elevated expression level of the HDS gene was not maintained after 24 hours of inoculation,potentially explaining the subsequent decrease in ginsenoside content.Our finding identified PMVK and HDS as two key enzyme genes that may play important roles in the chemical response of Panax notoginseng leaves to Botrytis cinerea infection.These results provide a basis for a deeper understanding of the interaction between Panax notoginseng and Botrytis cinerea,and reinforce the efforts towards the molecular breeding of Panax notoginseng resistant varieties.关键词
三七/病原胁迫/三萜皂苷/转录组测序/差异表达基因Key words
Panax notoginseng/pathogen stress/triterpenoid saponin/transcriptome sequencing/differentially expressed genes引用本文复制引用
焦晓林,殷梦奇,陈星星,徐江,郭帅,杨斌,马维思..三七叶片人参皂苷成分对灰葡萄孢菌侵染的响应及转录组分析[J].核农学报,2025,39(5):897-906,10.基金项目
北京市教育委员会科研计划项目(KM202311418001),中国中医科学院科技创新工程项目(CI2023E002),云南省重大科技计划(202202AE090001),云南省科技创新人才计划项目(202205AD160021) （KM202311418001）
