核农学报2025，Vol.39Issue(5)：916-926,11.DOI:10.11869/j.issn.1000-8551.2025.05.0916
基于转录组与代谢组分析核桃无融合生殖成熟胚的形成
Transcriptome and Metabolome Analysis of Walnut Apomixis Mature Embryo Formation
摘要
Abstract
In order to investigate the molecular regulatory mechanisms of apomictic embryo development in walnuts,the apomictic embryo(CL)and fertilized embryo(CK)of Xinxin 2 walnut were used as experimental materials in this study.The transcriptome and metabolome data were used to analyze the differentially expressed genes and differentially accumulated metabolites between CL and CK.The results indicated that a total of 227 differentially expressed genes and 22 differentially accumulated metabolites were identified in the CL and CK.Transcriptome-metabolome integrative analysis showed that the differentially expressed genes and metabolites were majorly enriched in pathways associated with metabolism,the biosynthesis of secondary metabolites and plant hormone signal transduction.Among them,the content of differential metabolites such as indole-3-acetic acid,jasmonic acid,and jasmonic acid-isoleucine were significantly higher in CL compared to CK.Moreover,genes such as(s)-genistein-9-o-methyltransferase(SMT),Chitinase(CHIT),Fumarylacetoacetase(FAH),Moxilactone-A synthase(MAS),Phospholipase A1(DAD1),Auxin response factor(ARF)and Phytochrome interaction factor 3(PIF3)were found to positively regulate these differential metabolites.On the other hands,genes including Cinnamyl alcohol dehydrogenase(MEE),Caffeic acid 3-O-methyltransferase(COMT),β-glucosidase(BGLU),Fatty acid α-dioxygenase(DOX),SAUR family protein(SAUR),Auxin response GH3 gene family(GH3),Ethylene receptor(ETR)and Transcription factor MYC2(MYC2)negatively regulated these metabolites.Eight genes that related to differentially metabolites were verified by quantitative real-time PCR(qRT-PCR),and the results were consistent with the transcriptome analysis.ARF,GH3,SAUR,DAD1 and MYC2 were involved in regulating apomictic embryo development in walnut by regulating plant hormone signal transduction,such as auxin and jasmonic acid pathway.These results can provide scientific guidance for understanding apomictic embrvo development in walnuts.关键词
核桃/无融合生殖/胚/转录组/代谢组Key words
walnut/apomixis/embryo/transcriptome/metabolome引用本文复制引用
宝春杰,周昊亮,陈凤,宋瑞龙,陈虹..基于转录组与代谢组分析核桃无融合生殖成熟胚的形成[J].核农学报,2025,39(5):916-926,11.基金项目
自治区基金自然科学基金项目(2022D01A180),国家自然科学基金项目(31960325) （2022D01A180）
