核农学报2025，Vol.39Issue(5)：934-943,10.DOI:10.11869/j.issn.1000-8551.2025.05.0934
低氧与凋亡相关因子在牦牛肠道的差异性分析
Differential Analysis of Hypoxia and Apoptosis-Related Factors in Intestinal Tract of Yaks
摘要
Abstract
To investigate the role of hypoxia in cell aging and apoptosis,small intestinal tissues(jejunum,ileum,and duodenum)of yaks were selected as materials.The differential expression of hypoxia-inducible factor 1α(HIF-1α),B-cell lymphoma 2(Bcl-2),Bcl-2 family-related protein Bax(Bax),caspase-3,and vascular endothelial growth factor(VEGF)in the tissues of different ages(newborn,5 months old,and adult)was analyzed.Immunohistochemical analyses were performed by collecting small intestine tissues from yaks of all ages with special stains including hematoxylin-eosin staining(H&E),povidone-iodine acid staining(PAS)and Masson trichrome staining(Masson).Results of H&E and special staining showed that the differences in histologic measures among the intestines were concentrated in the villi and glands of the duodenum,which was consistent with its role as the primary site of digestion.In addition,the highest collagen fiber content was observed in the jejunum of 5-months-old yaks.The results of immunohistochemistry showed that low-oxygen and apoptosis-related factors were differentially expressed in the duodenum as well.Hypoxia induced the transcription of HIF-1α activates apoptosis factors and induced cell apoptosis.As the age increased,yaks gradually adapted to the hypoxic environment,leading to a decrease in the expression of HIF-1α decreases.These findings provide basic data for further investigations into the distribution of HIF-1α,VEGF,Bax,Bcl-2 and Caspase-3 during the development of yak intestinal tract,and provide references for revealing the apoptosis-related factors involved in the hypoxic adaptation of the yak intestinal tract.关键词
牦牛/肠/低氧/凋亡Key words
yak/intestine/hypoxia/apoptosis引用本文复制引用
王军,廖艺媛,张凡,周熳琳,陈伟基,丁海娥,谈笑,杨琨..低氧与凋亡相关因子在牦牛肠道的差异性分析[J].核农学报,2025,39(5):934-943,10.基金项目
甘肃省自然科学基金项目(24JRRA155),兰州市青年科技人才创新项目(2023-QN-70),中央高校团队创新项目(31920230152),中央高校基本科研业务费专项资金资助(31920240127) （24JRRA155）
