饲料研究2025，Vol.48Issue(5)：1-7,7.DOI:10.13557/j.cnki.issn1002-2813.2025.05.001
不同粗蛋白水平颗粒料对断奶犊牛体外瘤胃发酵的影响
Effects of granular feed with different crude protein levels on in vitro rumen fermentation of weaned calves
摘要
Abstract
The aim of this experiment was to investigate the effects of different crude protein(CP)levels on in vitro rumen fermentation parameters and bacterial flora of weaned calves.In vitro rumen fermentation technique was used,and the crude protein levels were 16.0%,18.5%,21.0%and 23.5%granular feed as the substrate.The gas production,gas composition,fermentation parameters and rumen bacterial flora were determined by anaerobic culture for 48 h.The results showed that compared with CP level of 16.0%,the gas production at 24,36 and 48 h at CP level of 21.0%and 23.5%was significantly decreased(P<0.05),the unit hydrogen,methane and carbon dioxide production were significantly decreased(P<0.05),and the pH value and ammonia nitrogen(NH3-N)concentrations were significantly increased(P<0.05).The concentrations of microbial protein(MCP),isobutyric acid,and valeric acid at 23.5%CP level and the relative abundance of Proteobacteria were significantly increased(P<0.05).Compared with CP level of 16.0%,the relative abundances of Verrucococcus,Cyanobacteriota,Patescibacteria and Veillonella at CP level of 18.5%,21.0%and 23.5%were significantly decreased(P<0.05).Compared with the other three groups,the relative abundances of Ruminococcus,Eubacterium coprosterogenes and R-7 genera of Christensenaceae with CP level of 18.5%were significantly increased(P<0.05).The results show that increasing the dietary CP level of weaned calves can promote the synthesis of MCP,and lower CP level may promote rumen fiber degradation of weaned calves by increasing the relative abundance of fibrine-degrading bacteria such as Ruminococcus in the rumen,and the appropriate CP level of weaned calves is 21.0%considering the grain feed.关键词
断奶犊牛/粗蛋白水平/体外发酵/细菌区系Key words
weaned calves/crude protein level/in vitro fermentation/bacterial flora分类
畜牧业引用本文复制引用
宗玉洁,程志强,刘翁博洋,刘丽,赵静雯,乌日金,林淼..不同粗蛋白水平颗粒料对断奶犊牛体外瘤胃发酵的影响[J].饲料研究,2025,48(5):1-7,7.基金项目
江苏省研究生实践创新计划项目(项目编号:SJCX23_1994) （项目编号:SJCX23_1994）
国家奶牛产业技术体系(项目编号:CARS36) （项目编号:CARS36）
江苏省碳达峰碳中和科技创新专项资金(项目编号:BE2022309) （项目编号:BE2022309）
