饲料研究2025，Vol.48Issue(5)：73-77,5.DOI:10.13557/j.cnki.issn1002-2813.2025.05.013
响应面优化杜仲苷超声辅助提取工艺及其抗氧化活性研究
Study on optimization of ultrasonic assisted extraction process and antioxidant activity of Eucommia ulmoides glycosides using response surface methodology
摘要
Abstract
The experiment aimed to optimize the extraction process of Eucommia ulmoides glycosides and explore its antioxidant activity.Using ultrasound assisted water extraction method,Eucommia ulmoides leaves were used as raw materials,with liquid to material ratio,ultrasound temperature,and ultrasound time as independent variables,and Eucommia ulmoides glycosides extraction rate as the response value.The extraction process parameters were optimized by response surface analysis,and its microstructure and antioxidant activity were studied.The results showed that under the conditions of liquid-solid ratio of 19 mL/g,ultrasonic temperature of 69℃and ultrasonic time of 39 min,the extraction yield of Eucommia ulmoides glycosides was the highest(7.22%).The surface of Eucommia ulmoides glycosides was rough and had obvious void structure.The scavenging efficiency of 1,1-diphenyl-2-trinitrophenylhydrazide(DPPH)free radical,hydroxyl free radical and 2,2-diazo-bis(3-ethyl-benzothiazolin-6-sulfonic acid)diamiammonium salt(ABTS)free radical were 90.06%,77.62%and 100.00%,respectively,when the concentration of Eucommia ulmoides glycosides was 3.0 g/L.The study indicates that Eucommia ulmoides glycosides can be used as antioxidant feed additive in animal breeding.关键词
杜仲苷/提取工艺/响应面分析法/抗氧化Key words
Eucommia ulmoides glycosides/extraction process/response surface methodology/antioxidant分类
农业科技引用本文复制引用
曹雨欣,张雪蕾,赵传生,毕珂彤,王松山,张萌,吕培凡,张勇..响应面优化杜仲苷超声辅助提取工艺及其抗氧化活性研究[J].饲料研究,2025,48(5):73-77,5.基金项目
河南省高等学校重点科研项目(项目编号:24B230001) （项目编号:24B230001）
河南工业大学高层次人才引进项目(项目编号:2022BS072) （项目编号:2022BS072）
