饲料研究2025，Vol.48Issue(5)：78-83,6.DOI:10.13557/j.cnki.issn1002-2813.2025.05.014
基于网络药理学的益母草防控母畜产后炎症性疾病的分子机制研究
Study on molecular mechanism of Leonurus japonicas Houtt.in preventing and controlling postpartum inflammatory diseases in female livestock based on network pharmacology
摘要
Abstract
The study aimed to explore the molecular mechanism of Leonurus j aponicas Houtt.in preventing and controlling postpartum inflammatory diseases in female livestock using network pharmacology.Using Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)to screen active ingredients and potential targets of Leonurus j aponicas Houtt.,then mastitis,endometritis and vaginitis targets were obtained by GeneCards and OMIM databases.A protein-protein interaction(PPI)network was constructed using the STRING platform,and GO functional enrichment and KEGG enrichment analysis were carried out with DAVID database.Animal experiments were conducted to evaluate the effects of Leonurus j aponicas Houtt.on immune indicators in sows.The results showed that Leonurus j aponicas Houtt.contains 10 active components,involving 223 targets,with 1 023 intersection targets related to postpartum inflammatory diseases in livestock.GO enrichment analysis revealed 325 biological process entries,including positive regulation of plasma cell differentiation and B cell apoptosis,26 cellular component entries,including the extracellular region,chromatin,and membrane raft,and 75 molecular function entries,including cytokine activity and protein dimerization activity.KEGG pathway enrichment analysis identified 175 signaling pathways,including cancer,AGE-RAGE signaling pathway in diabetic complications,tumor necrosis factor signaling pathway,interleukin-17(IL-17)pathway,mitogen-activated protein kinase(MAPK)signaling pathway,and phosphatidylinositol 3-kinase-serine/threonine kinase(PI3K-Akt)signaling pathway,all of which are related to inflammation.Animal experiments demonstrated that the addition of Leonurus j aponicas Houtt.powder significantly reduced the levels of interleukin-2(IL-2),interleukin-2(IL-6),and tumor necrosis factor-α(TNF-α)in sow serum(P<0.05),and significantly increased the levels of immunoglobulin(IgA)and immunoglobulin(IgG)(P<0.05).The study indicates that Leonurus j aponicas Houtt.can prevent and control postpartum inflammatory diseases in livestock through active components such as quercetin,kaempferol,arachidonic acid,isorhamnetin,leonurine,and stachydrine,acting on targets such as tumor protein P53(TP53),TNF,IL-6,mitogen-activated protein kinase 14(MAPK14),and caspase-3(CASP3)via inflammation-related signaling pathways.关键词
益母草/母畜产后炎症性疾病/网络药理学/分子机制Key words
Leonurus japonicas Houtt./postpartum inflammatory diseases in female livestock/network pharmacology/molecular mechanism分类
农业科技引用本文复制引用
廖玲玲,宣泽义,陈少梅,曹艳红,奚玉莲,谢恒生,黄焕昌,胡湘云..基于网络药理学的益母草防控母畜产后炎症性疾病的分子机制研究[J].饲料研究,2025,48(5):78-83,6.基金项目
2023年广西农业科技自筹经费项目(项目编号:Z2023099) （项目编号:Z2023099）
