饲料研究2025，Vol.48Issue(5)：107-114,8.DOI:10.13557/j.cnki.issn1002-2813.2025.05.019
山香圆叶黄酮提取工艺优化及体外抑菌活性研究
Study on optimization of extraction process of total flavonoids from Turpiniae folium and in vitro antibacterial activity
摘要
Abstract
This study aimed to explore the optimal extraction process of total flavonoids from Turpiniae folium,quantitatively analyze the content of flavonoid monomers,and evaluate the in vitro antibacterial activity of the Turpiniae folium extract.Using Turpiniae folium as raw materials,the leaves were pre-treated with a ball mill to obtain activated Turpiniae folium powder.The reflux extraction method with ethanol as the solvent was employed.Based on single-factor experiments,an L16(45)orthogonal experiment was used to study the effects of ethanol concentration,extraction time,extraction temperature,liquid-solid ratio,and extraction frequency on the extraction rate of total flavonoids from Turpiniae folium,and the optimal extraction process was selected.The content of 35 flavonoid monomers in the freeze-dried powder of Turpiniae folium extract was analyzed using liquid chromatography-mass spectrometry.The in vitro antibacterial zone size was determined using the drug sensitivity disc method,and the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of the effective traditional Chinese medicine were measured using the tube dilution method.The results showed that the optimal extraction process for total flavonoids from Turpiniae folium was a liquid-solid ratio of 50 mL/g,extraction time of 1 h,ethanol concentration of 50%,extraction frequency of three times,and extraction temperature of 70℃,with a total flavonoid yield of 20.88 mg/g.A total of 25 flavonoid monomers were detected in the freeze-dried powder of the extract,with rutin having the highest content at 276.450 mg/g.Other monomers with content greater than 0.010 mg/g included quercetin,isoquercitrin,formononetin,kaempferol,astragalin,luteolin,biochanin A,genistein,and isovitexin,with contents of 1.769,1.040,0.085,0.061,0.053,0.016,0.016,0.015,and 0.015 mg/g,respectively.The extract was highly sensitive to Escherichia coli and Salmonella,and moderately sensitive to Staphylococcus aureus.Based on the total flavonoids from Turpiniae folium,the MIC values for Salmonella,Escherichia coli,and Staphylococcus aureus were 1.88,3.75,and 7.50 mg/L,respectively,and the MBC values were 7.50,15.00,and 15.00 mg/L,respectively.The study indicates that the established extraction process for total flavonoids from Turpiniae folium is stable and efficient.Rutin is the main flavonoid monomer in the extract,which has strong antibacterial activity.关键词
山香圆叶/总黄酮/提取工艺/正交试验/抑菌效果Key words
Turpiniae folium/total flavonoids/extraction process/orthogonal experiment/antibacterial effect分类
农业科技引用本文复制引用
熊平文,宋文静,陈将,宋琼莉,艾高祥,邹志恒,韦启鹏,陈小连..山香圆叶黄酮提取工艺优化及体外抑菌活性研究[J].饲料研究,2025,48(5):107-114,8.基金项目
江西省农业科学院基础研究与人才培养项目(项目编号:JXSNKYJCRC202216) （项目编号:JXSNKYJCRC202216）
江西省现代农业产业技术体系建设专项(项目编号:JXARS-12) （项目编号:JXARS-12）
江西省重点研发计划(项目编号:20232BBF60014、20224BBF62003) （项目编号:20232BBF60014、20224BBF62003）
赣鄱俊才支持计划高层次高技能领军人才培养项目(项目编号:2023) （项目编号:2023）
