生物骨科材料与临床研究2025，Vol.22Issue(2)：42-48,7.DOI:10.3969/j.issn.1672-5972.2025.02.008
人脐带间充质干细胞改善地塞米松诱导的骨微血管内皮细胞凋亡
hUCMSCs ameliorate dexamethasone-induced apoptosis of bone microvascular endothelial cells
朱正杰 1陈俊文 1徐瀚哲 1钟瑜健 1彭昊1
作者信息
- 1. 武汉大学人民医院关节外科,湖北 武汉,430060
- 折叠
摘要
Abstract
Objective To investigate the effect of hUCMSCs on Dex-induced bone microvascular endothelial cell(BMEC)function.Methods Bone microvascular endothelial cells were isolated and identified,and CCK8 was used to detect the effect of different concentrations of dexamethasone(Dex)on the viability of bone microvascular endothelial cells.The appropriate Dex experimental concentration was selected to treat bone microvascular endothelial cells to construct a cell model.The experiment was divided into blank group,Dex treatment group and Dex+hUCMSC treatment group(Blank group:BMECs cultured alone,Dex treatment group:BMECs cultured with dexamethasone,Dex+hUCMSC treatment group:BMECs cultured with dexamethasone and co-cultured with hUCMSC).The migration and invasion ability of bone microvascular endothelial cells in each group were evaluated by cell scratch test and Transwell test.Flow cytometry and Western blot were used to detect the apoptosis of bone microvascular endothelial cells in each group.Results ①The primary cells isolated and extracted in this study were identified as BMECs by immunofluorescence detection of co-expressing CD31 and VWF.②The results of the CCK8 test showed that Dex at a concentration of 200 μM significantly inhibited the viability of BMECs,which was set as the experimental concentration.③Scratch test and Transwell test:the migration and invasion ability of BMECs was significantly inhibited by Dex intervention,and the damage of migration and invasion ability of BMECs in Dex+hUCMSC treatment group was lower than that in Dex treatment group.④Apoptosis detection by Western blot and flow cytometry:Dex significantly induced the occurrence of apoptosis in BMECs.After co-culture with hUCMSCs,the apoptosis of BMECs induced by Dex was partially reversed.Conclusion hUCMSCs can alleviate the apoptosis of BMECs induced by dexamethasone,and also ameliorate the cell function damage induced by dexamethasone to a certain extent.关键词
人脐带间充质干细胞/激素性股骨头坏死/骨微血管内皮细胞细胞Key words
Human umbilical cord mesenchymal stem cells/Steroid-induced osteonecrosis of the femoral head/Bone microvascular endothelial cells分类
基础医学引用本文复制引用
朱正杰,陈俊文,徐瀚哲,钟瑜健,彭昊..人脐带间充质干细胞改善地塞米松诱导的骨微血管内皮细胞凋亡[J].生物骨科材料与临床研究,2025,22(2):42-48,7.
