微生物（英文）2025，Vol.4Issue(1)：70-82,13.DOI:10.1002/mlf2.12159
Developing a microfluidic-based epicPCR reveals diverse potential hosts of the mcrA gene in marine cold seep
Developing a microfluidic-based epicPCR reveals diverse potential hosts of the mcrA gene in marine cold seep
Wenli Shen 1Ziyan Wei 2Qing He 3Zhiyi Wang 2Kai Feng 3Wenbin Du 4Ye Deng 1Danrui Wang 5Jiangtao Li 6Yue Liu 7Yinzhao Wang 8Xingsheng Yang 3Xi Peng 3Bingliang Xie 2Lei Su6
作者信息
- 1. CAS Key Laboratory of Environmental Biotechnology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing,China||Institute for Marine Science and Technology,Shandong University,Qingdao,China
- 2. State Key Laboratory of Microbial Resources,Institute of Microbiology,Chinese Academy of Sciences,Beijing,China
- 3. CAS Key Laboratory of Environmental Biotechnology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing,China
- 4. State Key Laboratory of Microbial Resources,Institute of Microbiology,Chinese Academy of Sciences,Beijing,China||Savaid Medical School,University of the Chinese Academy of Sciences,Beijing,China
- 5. CAS Key Laboratory of Environmental Biotechnology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing,China||Soil Ecology Lab,Nanjing Agricultural University,Nanjing,China
- 6. State Key Laboratory of Marine Geology,Tongji University,Shanghai,China
- 7. College of Environmental Science and Engineering,Liaoning Technical University,Fuxin,China
- 8. Microbiology Division,School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai,China
- 折叠
摘要
关键词
anaerobic oxidation of methane/cold seep/epicPCR/mcrA geneKey words
anaerobic oxidation of methane/cold seep/epicPCR/mcrA gene引用本文复制引用
Wenli Shen,Ziyan Wei,Qing He,Zhiyi Wang,Kai Feng,Wenbin Du,Ye Deng,Danrui Wang,Jiangtao Li,Yue Liu,Yinzhao Wang,Xingsheng Yang,Xi Peng,Bingliang Xie,Lei Su..Developing a microfluidic-based epicPCR reveals diverse potential hosts of the mcrA gene in marine cold seep[J].微生物（英文）,2025,4(1):70-82,13.基金项目
This work was financially supported by the National Natural Science Foundation of China(Nos.32001092,91851106,and 91951103).We sincerely thank Professor Xiu-Zhu Dong for providing the strain Methanolobus psychrophilus R15,which was used for preliminary ex-periments.We gratefully thank James W.Voordeckers for his suggestions and grammar corrections on this paper.In addition,we sincerely thank Abby Deng for helping with the epicPCR experiments. （Nos.32001092,91851106,and 91951103）
