山西农业大学学报（自然科学版）2025，Vol.45Issue(2)：1-8,8.DOI:10.13842/j.cnki.issn1671-8151.202407042
基于全基因组重测序的五指山大叶茶种质资源多样性分析
Diversity analysis of germplasm resources of large-leaf tea in Wuzhishan mountains based on whole ge-nome resequencing
摘要
Abstract
[Objective]Wild large-leaf tea plants(Camellia sinensis)are widely distributed in the Wuzhishan region of Hainan Is-land and holds significant utilization value.However,there is limited research on its genetic background and genomic diversity.Based on the existing large-leaf tea plant genome,this study conducted the whole genome resequencing and germplasm re-source diversity analysis of wild large-leaf tea from representative regions in the central Wuzhishan area of Hainan.The aim was to provide a theoretical foundation for the conservation of Wuzhishan wild large-leaf tea plants and its application in tea plant genetic breeding.[Methods]In this study,69 representative samples of wild germplasms of tea plants in Wuzhishan were collected for whole-genome resequencing.Based on the resequencing data,principal component analysis(PCA),phylogenetic analysis,and genetic structure analysis were performed.Additionally,selective sweep sites were identified for large-leaf tea within the Shuiman Township.[Result]Whole genome resequencing of wild large-leaf tea generated a total of 60 001 469 742 bp of resequencing data.By aligning this data to the reference genome of large-leaf tea,41 381 015 variant sites were detected,including 39 970 726 SNP sites and 141 029 Indel sites.The distribution density trend of nucleotide polymorphism(π)values across the genome were found to be highly consistent with the distribution of SNPs and Indels.Principal component analysis,phylogenetic analysis,and genetic structure analysis collectively indicated that the wild large-leaf tea of Wuzhishan could be di-vided into five populations.Among these,populations B,C,and E exhibited relatively pure genetic backgrounds,while popu-lations A and D had more complex genetic backgrounds.The large-leaf tea resources within Shuiman Township were located in populations D and E,and some of the variant sites contributing to the differentiation between these two populations were distrib-uted across 2273 protein-coding genes.[Conclusion]Based on the whole genome re-sequencing data,multiple genetic diversity analyses revealed that Wuzhishan wild large-leaf tea can be classified into five populations,with the large-leaf tea from Shuiman Township distributed across two of these populations.This study provided a foundation for understanding the richness of Wu-zhishan large-leaf tea resources,formulating conservation strategies,and applying these findings in breeding programs.关键词
大叶茶/五指山/野生种质资源/重测序/遗传结构Key words
Camellia sinensis/Wuzhishan/Wild germplasm/Resequencing/Genetic structure分类
农业科学引用本文复制引用
吴海霞,苟志辉,杜尚嘉,符溶,符生波,符明珠,吴挺聪,陈国德,郭霞..基于全基因组重测序的五指山大叶茶种质资源多样性分析[J].山西农业大学学报（自然科学版）,2025,45(2):1-8,8.基金项目
五指山市科技项目(WZSKJXM2021005) （WZSKJXM2021005）
省属科研院所技术创新专项(SQKY2022-0037) （SQKY2022-0037）
