山西农业大学学报（自然科学版）2025，Vol.45Issue(2)：39-49,11.DOI:10.13842/j.cnki.issn1671-8151.202410034
沙棘混合油对小鼠慢性酒精肝损伤及肠道菌群的调节作用
Regulatory effects of sea buckthorn mixed oil on chronic alcohol-induced liver injury and intestinal mi-crobiota in mice
摘要
Abstract
[Objective]The current study was aimed to investigate the effects of sea-buckthorn mixed oil with a specific fatty acid ratio on chronic alcohol-induced liver injury and intestinal microbiota in mice.[Methods]Sea-buckthorn fruit oil and seed oil were mixed in a specific ratio to prepare sea-buckthorn oil mixtures with a saturated fatty acid(SFA):monounsaturated fatty acid(MUFA):polyunsaturated fatty acid(PUFA)ratio of 1∶1.41∶1.48.Mice were randomly divided into a normal control(NC)group,an ethanol model(AC)group,a high-dose mixed oil intervention(HM)group(750 mg/kg bw),a medium-dose mixed oil(MM)group(600 mg/kg bw),and a low-dose mixed oil group(LM)(450 mg/kg bw).A chronic alcoholic liver in-jury(ALD)model was established by increasing ethanol concentration consumption in mice.After six weeks of modeling by in-cremental ethanol gavage,18 biochemical indicators,including liver function,oxidative stress,and inflammatory factors,were measured.Hematoxylin-eosin(HE)staining was used to observe hepatic histopathological changes.Mouse feces were collect-ed for 16S rRNA sequencing to analyze intestinal microbiota diversity.[Results]Compared with the AC group,all three doses of sea buckthorn mixed oil intervention significantly alleviated chronic alcohol-induced hepatic steatosis,reduced liver function markers such as alanine transaminase(ALT)and aspartate transaminase(AST),reduced hepatic total triglyceride(TG),to-tal cholesterol(TC),low-density lipoprotein(LDL),and liver index levels,and lowered oxidative stress markers such as malondialdehyde(MDA)and peroxidase(MPO),as well as inflammatory factors such as interleukin-1(IL-1),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α).The intervention also reduced total bile acid(TBA)levels in the liver and fe-ces,while increasing high-density lipoprotein(HDL),superoxide dismutase(SOD)and glutathione(GSH)levels in the liver.The medium-dose intervention(MM)group exhibited the best effects,followed by the HM group.Intestinal microbiota analy-sis indicated that chronic alcohol exposure reduced β-diversity and altered microbial composition and function in mice.Sea buck-thorn mixed oil intervention increased β-diversity,enhanced the abundance of Akkermansia and Ruminococcus at the family lev-el,and decreased the functional abundance of Shigella.[Conclusion]Sea-buckthorn mixed oil with an SFA∶MUFA∶PUFA ra-tio of 1∶1.41∶1.48 can effectively alleviate chronic alcohol-induced liver injury in mice by mitigating oxidative stress,inflamma-tory factors,and modulating intestinal microbiota.关键词
特定饱和度脂肪酸配制/沙棘混合油/慢性酒精肝损伤/肠道菌群/胆酸Key words
Fatty acid preparation with specific saturation ratio/Sea-buckthorn oil mixture/Chronic alcoholic liver injury/Intes-tinal microbiota/Bile acid分类
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王芳,张滨,张鑫,赵普润,李博文,霍乃蕊,祁曣槿,秦旭巍,郭建峰..沙棘混合油对小鼠慢性酒精肝损伤及肠道菌群的调节作用[J].山西农业大学学报（自然科学版）,2025,45(2):39-49,11.基金项目
吕梁市重点研发项目(2023SHFZ03) （2023SHFZ03）
吕梁市校地合作重点研发专项(2023XDHZ06) （2023XDHZ06）
