山西农业大学学报（自然科学版）2025，Vol.45Issue(2)：50-59,10.DOI:10.13842/j.cnki.issn1671-8151.202411050
多糖EPS66A水剂研制及其对苹果花叶病的诱导抗性研究
Development of EPS66A polysaccharide aqueous formulation and its induced resistance to apple mosaic disease
摘要
Abstract
[Objective]The purpose of the current research was to explore the induced resistance of polysaccharide extract EPS66A from the fermentation broth of Streptomyces changanensis HL-66 against apple mosaic disease and to provide a scien-tific basis for the development of plant immune inducers to control plant diseases.[Methods]The preparation and quality evalua-tion of EPS66A aqueous solution were conducted according to national standards.Field efficacy trials were conducted to assess its control effect on apple mosaic disease.Physiological and biochemical indicators,such as antioxidant enzyme activities,were measured,and the expression levels of disease resistance-related genes were analyzed.[Results]The optimal formulation of the 1%EPS66A polysaccharide aqueous solution was:1%EPS66A polysaccharide,5%AES,1%carboxymethyl cellulose,1%ethylene glycol,1%B-263 defoamer,and water added to 100%.Field trial results indicated that the 200-fold dilution of the 1%EPS66A polysaccharide aqueous solution had the best control effect on apple mosaic virus disease,with a control efficacy of 73.99%at 15 days post-treatment.The activities of SOD,POD,and CAT in the leaves increased by 47.07%,70.34%,and 50.92%,respectively,compared to the control.The MDA content was 8.58 μmol/g,a 25.07%reduction compared to the control.qRT-PCR results showed that from 0 to 45 days after treatment with the 200-fold dilution of 1%EPS66A polysac-charide aqueous solution,the expression levels of antioxidant enzyme genes(CAT,SOD,and POD),flavonoid metabolic pathway genes(PAL1 and C4H),and pathogenesis-related protein genes(PR1,PR10a,and PR10b)in apple leaves were up-regulated.[Conclusion]The developed 1%EPS66A polysaccharide aqueous solution meets quality standards.It promoted the upregulation of disease resistance genes in apples,significantly enhanced antioxidant enzyme activities,reduced malondialde-hyde content,and enhanced the disease resistance of apple plants.It is an ideal inducer for controlling apple mosaic disease.关键词
免疫诱抗剂/苹果花叶病/田间防效/抗氧化酶/差异表达Key words
Immunostimulant/Apple mosaic disease/Field efficacy/Antioxidant enzymes/Differential expression分类
植物保护学引用本文复制引用
赵娟,张蕾,白格格,骞天佑,韩立荣..多糖EPS66A水剂研制及其对苹果花叶病的诱导抗性研究[J].山西农业大学学报（自然科学版）,2025,45(2):50-59,10.基金项目
国家自然科学基金项目(32272594) （32272594）
陕西省技术创新引导专项(2024QCY-KXJ-097) （2024QCY-KXJ-097）
