中国当代儿科杂志2025，Vol.27Issue(4)：472-479,8.DOI:10.7499/j.issn.1008-8830.2411067
2,6-DMBQ通过mTOR通路抑制支气管哮喘小鼠气道炎症反应的作用机制
Mechanism of 2,6-DMBQ attenuates airway inflammatory responses in asthmatic mice via the mTOR signaling pathway
摘要
Abstract
Objective To investigate the therapeutic effects and mechanisms of 2,6-dimethoxy-1,4-benzoquinone(2,6-DMBQ)in a mouse model of asthma.Methods SPF-grade BALB/c mice were randomly divided into 7 groups(n=8 each group):normal control group,ovalbumin(OVA)group,dimethyl sulfoxide+corn oil group,budesonide(BUD)group,and low,medium,and high dose 2,6-DMBQ groups.An asthma mouse model was established by OVA induction,followed by corresponding drug interventions.Non-invasive lung function tests were performed to measure airway hyperresponsiveness,and enzyme-linked immunosorbent assay was used to determine levels of interleukin(IL)-17,IL-10,and serum immunoglobulin E in bronchoalveolar lavage fluid.A cell counter was employed to detect eosinophil counts in bronchoalveolar lavage fluid,while hematoxylin-eosin staining and periodic acid-Schiff staining were used to assess lung tissue pathological changes.Western blot was conducted to examine the expression of proteins related to the mammalian target of rapamycin pathway(p-AKT/AKT and p-p70S6K/p70S6K),and a fully automated biochemical analyzer was used to evaluate liver and kidney functions.Results Compared with the normal control group,the OVA group showed increased enhanced pause values,inflammation scores from hematoxylin-eosin staining,positive area from periodic acid-Schiff staining,percentage of eosinophils,IL-17/IL-10 ratio,serum immunoglobulin E levels,and relative expression levels of p-AKT/AKT and p-p70S6K/p70S6K(P＜0.05).The BUD group and the medium and high dose 2,6-DMBQ groups exhibited decreased values for these indicators compared to the OVA group(P＜0.05).Conclusions 2,6-DMBQ can inhibit the mTOR pathway to alleviate airway inflammation in asthmatic mice,possibly by mitigating the imbalance between Th17 and regulatory T cells.关键词
支气管哮喘/2,6-二甲氧基-1,4-苯醌/哺乳动物雷帕霉素靶蛋白通路/Th17/Treg失衡/小鼠Key words
Bronchial asthma/2,6-Dimethoxy-1,4-benzoquinone/Mammalian target of rapamycin pathway/Th17/Treg imbalance/Mouse引用本文复制引用
李娟,李舒芳,熊晓曼,杨秋雁,谢雪丽,张艳丽..2,6-DMBQ通过mTOR通路抑制支气管哮喘小鼠气道炎症反应的作用机制[J].中国当代儿科杂志,2025,27(4):472-479,8.基金项目
河南省重点研发与推广专项(科技攻关)(232102310323). （科技攻关）
