同济大学学报（自然科学版）2025，Vol.53Issue(4)：635-643,9.DOI:10.11908/j.issn.0253-374x.23290
巴氏芽孢杆菌固化污染土的培养优化与矿化机制
Optimization of Sporosarcina pasteurii Culture and Mineralization for Soil Solidification
摘要
Abstract
The microbial-induced calcite precipitation(MICP)method represents an ecologically friendly and sustainable microbial treatment and remediation technology designed for soil solidification and contamination,and the efficacy of the method depends on the microorganism adept at urease production.A comprehensive investigation was conducted to study the impacts of factors such as oscillating speed,temperature,medium pH on the growth and urease activity of Sporosarcina pasteurii.The underlying mechanisms of biomineralization precipitation were revealed by synergistically utilizing techniques such as Fourier transformation infrared spectroscopy,X-ray photoelectron spectroscopy,X-ray diffraction,and scanning electron microscopy.The findings unequivocally demonstrate that an oscillating speed of 210 r-min-1 coincides with heightened levels of dissolved oxygen within the medium,thereby fostering elevated bacterial concentrations and augmented urease activity.The judicious utilization of an initial inoculum quantity amounting to 0.5％of the medium constituents engenders maximal nutrient consumption and precipitates rapid bacterial proliferation.A temperature of 30 ℃ is established as the ideal for fostering bacterial growth,striking a balance between avoiding dormancy due to lower temperatures and evading protein denaturation and inactivation attributed to excessive heat.The bacterial consortia exhibit a notable resilience across a pH spectrum spanning 5 to 10,with acidic conditions eliciting a prolonged interval for urease activity to attain its zenith.Despite this delay,the pinnacle of urease activity remains relatively unaltered,whereas an alkaline milieu is conducive to accentuating urease activity.Urea,as a pivotal determinant,exerts a direct influence on the adenosine triphosphate(ATP)synthesis,which is crucial for bacterial energy metabolism,and thus profoundly affecting both bacterial proliferation and urease activity.Notably,a urea concentration ranging from 5 to 25 g·L-1 fosters expedited bacterial growth rates coupled with heightened urease activity.The MICP process was carried out on extracellular polymers of bacteria,and the generated calcium carbonate was mainly composed of rhombic calcite and a small amount of spherical vaterite.关键词
微生物诱导碳酸钙沉淀(MICP)/污染土固化/巴氏芽孢杆菌/尿素/矿化机理Key words
microbial-induced calcite precipitation(MICP)/contaminated soils solidification/Sporosarcina pasteurii/urea/mineralization mechanism分类
建筑与水利引用本文复制引用
陈永贵,江昭明,付俊,周罕,文子豪..巴氏芽孢杆菌固化污染土的培养优化与矿化机制[J].同济大学学报（自然科学版）,2025,53(4):635-643,9.基金项目
国家自然科学基金(42125701) （42125701）
云南省万人计划产业技术领军人才科技项目(云发改人事[2019]274号) （云发改人事[2019]274号）
中铝国际重点科研项目(CJ2021JS-06) （CJ2021JS-06）
