针刺研究2025，Vol.50Issue(4)：393-401,9.DOI:10.13702/j.1000-0607.20240109
电针调控下丘脑LKB1/AMPK信号通路对肥胖小鼠白色脂肪棕色化的影响
Electroacupuncture intervention promotes browning of white adipose tissue by regulating LKB1/AMPK pathway in obese mice
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA)on hypothalamic liver kinase B1(LKB1)/AMP-activated protein kinase(AMPK)signaling pathway,sympathetic nerve activity and browning of white adipose tissue(WAT)in obese mice,so as to explore the possible central mechanisms of EA in improving obesity.Methods C57BL/6J male mice were divided into normal group(n=8)and high fat diet group(n=30)randomly,and fed by normal diet and high fat diet for 8 weeks,respectively.In the high fat diet group,mice with body weight over 20%heavier than that of the normal group were considered as obese mice.The obese mice were divided into model and EA groups(n=8 in each group).Mice in the EA group were treated with EA(2 Hz/15 Hz,1 mA,3 mm needling depth)at"Zusanli"(ST36)and"Tianshu"(ST25)for 30 min,5 times a week for 4 weeks.The mice's body weight and length were measured for calculating the Lee's index.The contents of serum triglyceride(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C)and noradrenaline(NE)were detected by ELISA.The weight of abdominal white adipose tissue was weighed,and the morphological changes of abdominal adipose tissue were observed by HE staining.The mRNA and protein expression levels of LKB1 and AMPK in hypothalamus,peroxisome proliferator-activated receptor γ coactivator 1α(PGC-1α),uncoupling protein-1(UCP1),peroxisome proliferator-activated receptor γ(PPAR-γ)and β3-adrenergic receptor(β3-AR)in abdominal white adipose tissue were detected by real-time fluorescence quantitative PCR and Western blot,respectively.Result Compared with the normal group,the body weight,Lee's index,serum contents of TC,TG and LDL-C,abdominal WAT mass,hypothalamus LKB1,AMPK mRNA and protein expression were increased(P<0.01),while serum HDL-C and NE contents,mRNA and protein expression of β3-AR,PGC-1α,UCP1 and PPAR-γ in adipose tissue were decreased(P<0.01)in the model group.The diameter of adipocytes in abdominal WAT was significantly increased,and the number of cells per unit area was decreased in the model group.Following EA intervention,the body weight,Lee's index,serum contents of TC,TG and LDL-C,abdominal WAT mass,hypothalamus LKB1,AMPK mRNA and protein expression were decreased(P<0.01),while the serum HDL-C and NE contents,mRNA and protein expression of β3-AR,PGC-1α,UCP1 and PPAR-γ were increased(P<0.01).The diameter of adipocytes in abdominal WAT was decreased and the number of cells per unit area was increased in the EA group.Conclusion EA can reduce the body weight and Lee's index in obese mice,regulate blood lipids,and reduce the weight of abdominal WAT,which may be closely related to its effect in regulating the hypothalamic LKB1/AMPK signaling pathway,activating the adipose tissue sympathetic nerve activity,and promoting the browning of WAT.关键词
肥胖/电针/白色脂肪棕色化/LKB1/AMPK信号通路/交感神经Key words
Obesity/Electroacupuncture/Browning of white adipose tissue/LKB1/AMPK pathway/Sympathetic nerve引用本文复制引用
张英溶,张艳佶,黄伟,陈霞,王佳捷,周仲瑜..电针调控下丘脑LKB1/AMPK信号通路对肥胖小鼠白色脂肪棕色化的影响[J].针刺研究,2025,50(4):393-401,9.基金项目
武汉市科技局知识创新专项项目(No.2023020201010172) （No.2023020201010172）
湖北省第二届医学领军人才工程培养对象暨湖北名医工作室项目(No.鄂卫通[2019]47号) （No.鄂卫通[2019]47号）
第七批全国老中医药专家学术经验继承项目(No.国中医药办人教函[2021]272号) （No.国中医药办人教函[2021]272号）
国家中医药管理局全国名老中医(王华教授)传承工作室(No.国中医药人教函[2022]75号) （王华教授）
国家中医药管理局"2020年中医药古籍文献和特色技术传承专项"(No.0686-204001171345N) （No.0686-204001171345N）
湖北中医药大学"双一流"建设重点类专项科研项目 （）
