中国真菌学杂志2025，Vol.20Issue(1)：45-51,7.
铁离子在耳念珠菌热抗逆中功能及机制初探研究
A preliminary study on the effect of iron on heat resistance mechanism in Candida auris
摘要
Abstract
Objective To explore the biological effect and molecular mechanism of iorn in heat resistance of Candida auris isolate.Methods Firstly,the OD600 growth curves of C.auris and C.hemulosaurus strains were measured under different temperatures and concentrations of iron chelator bathophenanthroline disulfonate in liq-uid medium treatment.Secondly,solid spot plate experiments at different temperatures and con-centrations of iron were performedin phenotypic analysis of C.auris and C.hemulosaurus strains.Thirdly,DCFH-DA fluorescent probe was used to determine the production of reactive oxygen species(ROS)in C.auris strain under different temperatures and concentrations of iron.Fourth-ly,the changes of glutaredoxin 5 gene(GRX5)expression under the conditions of different tem-peratures,concentrations of iron or H2O2 addition were detected by real-time PCR method.Results Compared with the C.hemulosaurus strain,C.auris strain showed stronger heat tolerance.The growth of C.auris strain was significantly promoted in the solid medium enriched with high con-centration of iron.In addition,the addition of iron chelator bathophenanthroline disulfonate would inhibit the ROS production of C.auris strain,while the enrichment of iron would promote the ROS production.Further more,real-time PCR analysis showed that the expression of GRX5 gene of C.auris strain was changed under different temperatures and concentrations of iron.Conclusion Iron is a neces-sary factor for the thermal stress resistance of C.auris strain.Moreover,the expression GRX5 gene is up-regulated to resist ROS stress caused by heat stress.关键词
耳念珠菌/希木龙念珠菌/铁离子/热抗逆/单巯基谷氧化还原蛋白基因Key words
Candida auris/Candida hemulosaurus/iron/heat resistance/glutaredoxin gene分类
基础医学引用本文复制引用
文欢,王也,黄雎,周浩,王棣,宋章永..铁离子在耳念珠菌热抗逆中功能及机制初探研究[J].中国真菌学杂志,2025,20(1):45-51,7.基金项目
四川省自然科学基金项目(2025ZNSFSC0602) （2025ZNSFSC0602）
泸州市-西南医科大学联合项目(2023LZXNYDJ031) （2023LZXNYDJ031）
叙永县人民医院-西南医科大学科技战略合作项目(2023XYXNYD08) （2023XYXNYD08）
临床疾病分子诊断泸州市重点实验室科技项目(FZZD2023-07) （FZZD2023-07）
