杂交水稻2025，Vol.40Issue(2)：45-51,7.DOI:10.16267/j.cnki.1005-3956.20240318.088
三抗籼型水稻光温敏核不育系华6421S的选育及应用
Breeding and Application of Indica Rice PTGMS Line Hua 6421S with Blast,Brown Planthopper and Bacterial Blight Resistances
摘要
Abstract
Hua 6421S is a photo-thermo-sensitive genic male sterile(PTGMS)line of indica rice,which was selected from the offspring of two PTGMS lines Hua 1015S and Hua 1165S and polymerized four resistance genes of Pi2,Xa23,Bph14 and Bph15 by molecular marker-assisted selection(MAS).The field identifying results showed that the incidence of both leaf blast and panicle neck blast was grade 3,and the indoor seedling blast identifying results of 33 rice blast strains showed that the resistance frequency was 94％.Three bacterial blight pathogens were inoculated artificially and the lesion length was 0.3～1.7 cm,indicating that it is resistant to bacterial blight.Identification of resistance to brown planthopper at seedling stage showed that its resistance level is grade 3,indicating it is resistant to brown planthopper.Under the conditions of light length 14.5 h and daily average temperature 23 ℃(night minimum temperature 19 ℃,daily maximum temperature 26 ℃)in the artificial climate chamber,the pollen sterility was 99.51％and self-fertilization sterility was 100％.The stable sterility period was 62 days from July 15 to September 15 under natural conditions in Wuhan.When seeded between May 10 and June 5,2018 in Wuhan,the growth duration from seeding to initial heading was 86～82 days,the effective panicle number per plant was 9.0,plant height was 80.3 cm,panicle length was 23.5 cm and the total spikelet number per panicle was 140.6.When seeded between November 20 and December 10,2018 at Lingshui County of Hainan Province,the growth duration from seeding to initial heading was about 85 days,The effective panicle number per plant was 12.1,plant height was 85.1 cm,panicle length was 19.4 cm,the total spikelet number per panicle was 147.5,the natural seed-setting rate was 85.28％and 1 000-grain weight was 25.4 g.The grain quality of rice harvested in Hainan was tested for two years.The average value of each index was 78.4％of brown rice rate,69.0％of milled rice rate,58.6％of head rice rate,2.7％of chalkiness degree,12.0％of chalky grain rate,6.0 mm of grain length,2.7 of length-width ratio,12.9％of amylose content,grade 6.3 of alkali spreading value,82 mm of gel consistency and grade 1 of transparency.It was approved by Hubei Crop Variety Appraisal Committee in July 2021.Derived from Hua 6421S,two two-line hybrid rice varieties with three resistances(resistance to rice blast,resistance to bacterial blight and resistance to brown planthopper)and one two-line hybrid rice variety with resistance to rice blast and resistance to bacterial blight have been developed and approved by Hubei Crop Variety Appraisal Committee.关键词
杂交水稻/光温敏核不育系/华6421S/稻瘟病抗性/白叶枯病抗性/褐飞虱抗性/分子标记选择/选育Key words
hybrid rice/PTGMS line/Hua 6421S/resistance to rice blast/resistance to bacterial blight/resistance to brown planthopper/MAS/breeding分类
农业科技引用本文复制引用
牟同敏,李春海,杨大兵,米甲明,熊立仲..三抗籼型水稻光温敏核不育系华6421S的选育及应用[J].杂交水稻,2025,40(2):45-51,7.基金项目
国家自然科学基金项目(31821005) （31821005）
